Supplemental Table 2 for:

Article Title: Effect of form of gestational Selenium on mRNAs encoding steroidogenesis and spermatogenesis in the neonatal calf testis

Journal Name: Biological Trace Element Research

Authors: K.L. Cerny, S. Garbacik, C. Skees, W.R. Burris, J.C. Matthews and P.J. Bridges

Affiliation: Department of Animal and Food Sciences, University of Kentucky, Lexington KY 40546

Corresponding author: P.J. Bridges, Ph.D.;

Supplemental Table 2 Canonical pathway analysis on the effect of form of supplemental selenium (Se) on the transcriptome of the neonatal calf testis a

Canonical Pathway b / P-value / Molecules c
Dermatan Sulfate Biosynthesis (Late Stages) / 0.0005 / SULT4A1,SULT1E1,CHST7,HS3ST2,HS6ST3,CHST10,DSE
Xenobiotic Metabolism Signaling / 0.001 / FTL,MAP3K11,CAMK4,CAMK1D,CHST7,GSTA4,PPP2R5B,NQO1,ALDH9A1,CYP2C19,PRKCI,SULT1E1,SULT4A1,MAPK14,PRKCD,HS3ST2,HS6ST3,CHST10,PPP2R1B,ALDH6A1
Dermatan Sulfate Biosynthesis / 0.003 / SULT4A1,SULT1E1,CHST7,HS3ST2,HS6ST3,CHST10,DSE
Chondroitin Sulfate Biosynthesis (Late Stages) / 0.004 / SULT4A1,SULT1E1,CHST7,HS3ST2,HS6ST3,CHST10
Valine Degradation I / 0.004 / BCAT2,DBT,ALDH6A1,BCKDHB
Estrogen Biosynthesis / 0.004 / AKR1C3,HSD17B7,CYP2J2,HSD17B4,CYP2C19
L-carnitine Biosynthesis / 0.005 / TMLHE,ALDH9A1
Bile Acid Biosynthesis, Neutral Pathway / 0.007 / BAAT,AKR1C3,SCP2
Tryptophan Degradation III (Eukaryotic) / 0.007 / L3HYPDH,TDO2,ACAT1,HSD17B4
Heparan Sulfate Biosynthesis (Late Stages) / 0.007 / SULT4A1,SULT1E1,CHST7,HS3ST2,HS6ST3,CHST10
Chondroitin Sulfate Biosynthesis / 0.009 / SULT4A1,SULT1E1,CHST7,HS3ST2,HS6ST3,CHST10
Glutaryl-CoA Degradation / 0.009 / L3HYPDH,ACAT1,HSD17B4
Branched-chain α-keto acid Dehydrogenase Complex / 0.010 / DBT,BCKDHB
Heparan Sulfate Biosynthesis / 0.013 / SULT4A1,SULT1E1,CHST7,HS3ST2,HS6ST3,CHST10
Serine Biosynthesis / 0.015 / PSAT1,DUSP26
Mechanisms of Viral Exit from Host Cells / 0.019 / PRKCI,PRKCD,ACTG1,CHMP3,ACTA1
Acetyl-CoA Biosynthesis I (Pyruvate Dehydrogenase Complex) / 0.022 / PDHA1,DBT
Sperm Motility / 0.023 / PLCB4,CAMK4,PRKCI,MAP3K11,PRKCD,PLCG2,NPR1,PLA2G4B, PDE1B
CCR3 Signaling in Eosinophils / 0.025 / PLCB4,CAMK4,PRKCI,MAPK14,PAK3,PRKCD,PLA2G4B,CCL24, GNG7
UVC-Induced MAPK Signaling / 0.026 / PRKCI,MAPK14,ARAF,PRKCD,SMPD3
Superpathway of Serine and Glycine Biosynthesis I / 0.031 / PSAT1,DUSP26
Primary Immunodeficiency Signaling / 0.033 / RFXANK,RFXAP,RAG1,CD79A
CCR5 Signaling in Macrophages / 0.036 / CAMK4,PRKCI,MAPK14,PRKCD,PLCG2,GNG7
Melatonin Signaling / 0.039 / PLCB4,CAMK4,PRKCI,ARAF,PRKCD,PLCG2
Citrulline Degradation / 0.041 / OTC
Chemokine Signaling / 0.042 / PLCB4,CAMK4,MAPK14,CAMK1D,PLCG2,CCL24
α-Adrenergic Signaling / 0.044 / CAMK4,PRKCI,ADRA2A,PRKCD,PLCG2,GNG7,GYS2

a Se supplement treatments contained 3 mg Se/day in the form of sodium selenite (ISe), SelPlex® (OSe) or 1.5 mg Se/day of each sodium selenite and SelPlex® (MIX)

b Canonical pathways were derived by subjecting differentially expressed genes (DEG) to Ingenuity Pathway Analysis (IPA®, QIAGEN Redwood City, www.qiagen.com/ingenuity)

c Molecules in each canonical pathway that were differentially expressed