Session VI – Plant Breeding, Germplasm Utilization and Cereal Genomics – Poster VI-25

QTLs Associated with Breeding Characteristics in Oat DH Mapping Population

Pirjo Tanhuanpää1), Elina Kiviharju1)*, Outi Manninen1), Alan Schulman1,2), Ruslan Kalendar2), Merja Eurola1), Veli Hietaniemi1), Marja Jalli1) and Leena Pietilä3)

1) MTT Agrifood Research Finland, 31600 Jokioinen, Finland

2) MTT/BI Plant Genomics Laboratory, Institute of Biotechnology, P.O. Box 56, 00014 University of Helsinki, Finland

3) Boreal Plant Breeding Ltd, Myllytie 10, 31600 Jokioinen, Finland

*Corresponding Author: PH: (358) 3-41881; E-mail:

Abstract

All the maps reported for hexaploid oat have been constructed using recombinant inbred lines (RILs). Another permanent population type is a doubled haploid (DH) population. Compared to RILs, DHs contain no residual heterozygosity. DHs have been applied for mapping in several cereal species, but in oats production of a DH mapping population has just recently started to be an option.

In this study, a mapping population of 137 DH plants was generated through anther culture of F1 plants from the spring oat cross Aslak ´ Matilda, by a method lately developed in MTT Agrifood Research Finland. Aslak is a Finnish cultivar of Boreal Plant Breeding Ltd and Matilda a Swedish cultivar of Svalöf Weibull AB.

We were particularly interested in finding chromosomal regions associated with b-glucan and oil content and oat leaf blotch resistance, but also several other traits were measured. Field tests were carried out during years 2005 (1 m2 plots with four replicates) and 2006 (6 m2 plots with three replicates). Resistance of the lines to Pyrenophora avenae was evaluated in a greenhouse test and b-glucan and oil content analysed from the yield of the field tests.

A variety of PCR-based DNA markers was analysed in the DH progeny, including microsatellites, RAPDs (random amplified polymorphic DNAs), REMAPs (retrotransposon-microsatellite amplified polymorphisms), ISSRs (inter simple sequence repeats), SRAPs (sequence-related amplified polymorphisms), and AFLPs (amplified fragment length polymorphisms).

A linkage map of 28 linkage groups consisting of 625 markers was built. The amount of markers in groups ranged from 6 to 70, and the total size of the map was 1526 cM. Seven QTLs were associated with oil content, two with b-glucan and several with leaf blotch disease resistance. In addition, QTLs associated with agronomical traits were located. Results from the QTL analyses will be shown and discussed. Our final goal was to provide marker-assisted tools for more efficient oat breeding.

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