How Do Gene and Chromosomal Mutations Affect Proteins?

  1. If the sense strand of DNA is AATGCCAGT, what is the base sequence of the antisense strand?
  1. Write the base sequence that would appear on a mRNA strand after transcription.
  1. Using figure 10-12 on page 189, determine the order of amino acids that would be produced.
  1. A point mutation is a change in a single base pair in DNA. If this changed the sense strand of DNA to AATCCCAGT, what would the resulting mRNA look like? What amino acids would be produced?
  1. A frameshift mutation occurs when a single base is added or deleted from the sense strand of DNA. If this changed the sense strand of DNA to AAATGCCAGT, what would the resulting mRNA look like? What amino acids would be produced?
  1. Deletions occur when a part of a chromosome is lost. If this changed the sense strand of DNA to AAGCCAGT, what would the resulting mRNA look like? What amino acids would be produced?
  1. Insertions occur when a part of a chromatid breaks off and attaches to its sister chromatid. The result is a duplication of genes on the same chromosome. If this changed the sense strand of DNA to AATGCCGCCAGT, what would the resulting mRNA look like? What amino acids would be produced?
  1. Inversions occur when a part of a chromosome breaks off, and is reinserted backwards. If this changed the sense strand of DNA to AATCCGAGT, what would the resulting mRNA look like? What amino acids would be produced?
  1. Translocations occur when part of one chromosome breaks off, and joins to another chromosome. If this changed the sense strand of DNA to AATGCCAGTAGCTCG, what would the resulting mRNA look like? What amino acids would be produced?
  1. If any of the above mutations occurred in an intron of the gene, what would we expect to happen? Explain.