On-Line Supplementary Data

On-line Supplementary Data

Table S1Tissue distribution of the CAPN3 mRNA at the same growth point

Growth point / Breast muscle / Heart / Liver / Brain / Leg muscle
0 week / 301.34A±45.13 / 9.23B±0.84 / 1.30B±0.09 / \ / 467.61A±65.25
2 weeks / 356.06A±36.59 / 3.18C±0.97 / 1.18C±0.93 / 0.59C±0.08 / 99.67B±6.59
4 weeks / 1794.76A±89.31 / 6.14C±0.99 / 1.11C±0.64 / 1.36C±0.78 / 1013.09B±76.03
6 weeks / 1563.31A±213.97 / 2.62C±0.99 / 1.26C±0.95 / 0.94C±0.65 / 842.98B±112.42
8 weeks / 555.68A±16.44 / 2.00B±0.87 / 1.08B±0.42 / 0.65B±0.21 / 747.59A±16.92
10 weeks / 647.39A±19.58 / 2.81B±0.97 / 1.21C±0.48 / 0.72C±0.32 / 298.47A±9.85
12 weeks / 926.92A±25.14 / 3.25B±0.98 / 1.24B±0.78 / 0.94B±0.41 / 744.14A±21.73

Note: Values in a line without a common uppercase mean that the relative quantities of the CAPN3gene differ significantly (P <0.01).Expression data were based on Sichuan Mountainous Black-boned chicken (MB) and were normalized to the endogenous ACTBexpression in the same tissue.

Table S2Ontogenic expression of the chicken CAPN3 mRNA

Week / Breast muscle / Heart / Liver / Brain / Leg muscle
0w / 0.26b±0.04 / 2.24a±0.55 / 0.86b±0.08 / \ / 0.80b±0.20
2w / 1.31a±0.39 / 3.94a±0.68 / 4.07a±0.62 / 2.34a±0.23 / 0.88b±0.21
4w / 1.71a±0.40 / 1.98a±0.28 / 0.98b±0.32 / 1.39b±0.13 / 2.32a±1.22
6w / 1.05a±0.93 / 0.60b±0.14 / 0.79b±0.07 / 0.68b±0.07 / 1.36b±0.42
8w / 1.48a±0.23 / 1.81a±0.27 / 2.68a±1.64 / 1.86a±0.26 / 4.81a±0.88
10w / 0.94a±0.07 / 1.38b±0.45 / 1.63b±0.13 / 1.12b±0.13 / 1.04b±0.56
12w / 1.06a±0.19 / 1.05b±0.56 / 1.14b±0.51 / 1.04b±0.21 / 1.60b±0.72

Note: Values in a column without a common lowercase mean that the relative quantities of the CAPN3gene in a tissue at different growth points differ significantly (P < 0.05). Expression data were based on Sichuan Mountainous Black-boned chicken (MB) and were normalized to the endogenous ACTBexpression in the same tissue.The expression level of the CAPN3 genein different tissues at 12weeks was arbitrarily setto 1.0.