From,

Dr.SHASHIKALA C. GUDAGERI, I M.D. (AYU) Scholar

Department of Post Graduate Studies In Rasashastra,

Taranath Government Ayurvedic Medical College,

BELLARY-583 101.

To,

THE REGISTRAR,

Rajiv Gandhi University of Health Sciences, Karnataka

BANGALORE.

Through

The Principal and

Head of the Department of Post Graduate Studies in Rasashastra

Taranath Government Ayurvedic Medical College

BELLARY-583 101.

Respected Sir,

Sub: Submission of completed Proforma for registration of Subject of Dissertation.

I request you to kindly register the below mentioned subject against my name for the submission of dissertation to the Rajiv Gandhi University of Health Sciences, Bangalore for the partial fulfillment of M.D (Ayurveda).

TITLE OF THE DISSERTATION:

“PHARMACEUTICO - ANALYTICAL STUDY OF KAMADUGHA RASA AND ITS ANTI -ULCER ACTIVITY ON ALBINO RATS-AN EXPERIMENTAL STUDY”

Here with I am enclosing completed Proforma for registration of subject for dissertation.

Thanking You Sir,

Yours faithfully,

Place:Bellary

Date: Dr.SHASHIKALA C.GUDAGERI

RAJIV GANDHI UNIVERSITY OF HEALTH SCIENCES, KARNATAKA,

BANGALORE

ANNEXURE-І І

PROFORMA FOR REGISTRATION OF SUBJECT FOR DISSERTATION

  1. Name of the candidate and address : Dr. SHASHIKALA C.GUDAGERI, I MD (AYU) Department of Post Graduate Studies in Rasashastra

Taranath Govt Ayurvedic Medical College

BELLARY-583101

Dr.SHASHIKALA C. GUDAGERI

D/O C.G. GUDAGERI

PLOT NO. 28

SAI NAGAR

KELGERI ROAD

DHARWAD - 580001

  1. Name of the Institution : TARANATH GOVERNMENT

AYURVEDIC MEDICAL COLLEGE,BELLARY-583101

  1. Course of Study and Subject : 1st Year M.D(Ayu)Rasashastra
  1. Date of admission to course : 23rd Nov 2009
  1. Title of the Topic :

“PHARMACEUTICO - ANALYTICAL STUDY OF KAMADUGHA RASA

AND ITS ANTI – ULCER ACTIVITY ON ALBINO RATS - AN

EXPERIMENTAL STUDY”.

6 BRIEF RESUME OF THE INTENDED WORK

6.1 Need for the study

Science is not stagnant; it is always a continuous process. It needs scientific validation. Ayurveda is ancient Indian art and science of healing and rejuvenation. Rasashastra is the offshoot of Ayurveda. In Rasashastra different methods of unique preparations are mentioned among them khalveeya rasayana is one.

Khalvi method is a unique method applicable to most of the rasoushadhies. It brings specific changes in the minerals which accelerates the therapeutic action and help in better absorption.

Kamadugha Rasa is one such important formulation containing Mukta Bhasma, Pravala Bhasma,Mukta Shukti Bhasma, Varatika Bhasma, Shankha Bhasma, Shuddha Gairika, Guduchi Satva. It is indicated in Amlapitta, Pittaja vikara, Jirna Jwara, Bhrama1.It is also advised in Vruna due to Amlapitta2.

Peptic ulcer is a world wide problem. Peptic ulcers are the areas of degeneration and necrosis of gastrointestinal mucosa exposed to acid-peptic secretions. Though they can occur at any level of the alimentary tract that is exposed to hydrochloric acid and pepsin, they occur most commonly (98-99%) in either the duodenum or the stomach in the ratio of 4:13. Current statistical data shows more than 70% of people are suffering from gastro-intestinal disturbances.

For the purpose of globalization of Rasoushadhies, preclinical methods like experimental study are very essential.Hence keeping the above as asset this study entitled “PHARMACEUTICO - ANALYTICAL STUDY OF KAMADUGHA RASA AND ITS ANTI –ULCER ACTIVITY ON ALBINO RATS - AN EXPERIMENTAL STUDY” is undertaken.

6.2 REVIEW OF LITERATURE

  1. Preparation of Kamadugha Rasa will be reviewed as per different Ayurvedic texts.
  2. Review of Shodhana of Mukta4, Pravala5, Mukta Shukti6, Varatika7, Shankha8and Gairika9.
  3. Review of Marana of Mukta10, Pravala11, Mukta Shukti12, Varatika13and Shankha14.
  4. Review of Preparation of Kamadugha Rasa1.
  5. Review of concept of Amlapitta, Parinama shoola,annadrava shoola and their treatment as per Ayurvedic classics.
  6. Review of concept of Peptic ulcer as per modern science.

Previous Works published in Relation to the Subject:

  1. Dr. Garga A. K, Kamadudha Rasa Kalpanayen uska Pitta Rogon Par Prabhavatmaka Adhyayana. (Doctoral dissertation, M.M.M. Govt. Ayurvedic College Rajasthan University, Jaipur 1982).
  2. Dr. Tyagi R. S, Kamadudha Rasa Nirmana aur Kamala Per Adhyayana. (Doctoral dissertation, Govt. Ayurvedic College, Baba Farid University of Health Sciences, Faridkot1983).

6.3 OBJECTIVES OF THE STUDY

  1. To carryout Shodhana of Mukta, Pravala, Mukta Shukti, Varatika, Shankha and Gairika.
  2. To carryout Marana of Mukta, Pravala, Mukta Shukti, Varatika and Shankha.
  3. To prepare Kamadugha Rasa.
  4. To do physico-chemical analysis of Kamadugha Rasa
  5. To evaluate anti ulcer activity of Kamadugha Rasa on albino rats by Indomethacin(20mg/kg) induced ulcer method.

7. MATERIALS AND METHODS

7.1 Source of data:

Literary:

All the relevant classical and up to date literature of Kamadugha Rasaand its

ingredients will be screened.Experimental anti ulceractivity study will be reviewed as per

modernPharmacology.

Pharmaceutical:

Genuine raw materials will be collected from authentic source.These will

be screened for Grahya lakshanas.Necessary processing of raw materials will be done in

Pharmacy section of P.G.Department of Rasashastra,in Taranath Govt.Ayurvedic

MedicalCollege, Bellary.

Analytical:

Kamadugha Rasa will be taken for physico-chemical analysis with

collaborationof accredited laboratory.

Experimental:

Albino rats will be usedfor experimental study.Ranitidine Hydrochloride

willbe used orally for the standard group.Animal experimental study will be carried out

at thedepartment of Pharmacology,Vijayanagar Institute of Medical Sciences (VIMS),

Bellary and the albino rats required for thestudy willbe obtained fromThe Central

AnimalHouse,VIMS,Bellary.

7.2 METHODS OF COLLECTION OF DATA

Pharmaceutical Study:

  • The raw materials will be screened and data will be collected.
  • Purification of Mukta will be done by subjecting it to swedana process in dolayantra with agastya swarasafor 1 yama4.
  • Purification of Pravala will be done by subjecting it to swedana process in dolayantra with sarjika ksharajala for 1 yama5.
  • Purification of Shukti6, Varatika7,Shankha8 will be done by subjecting it to swedana in dolayantra with nimbuswarasa.
  • Purification of Gairika will be done by subjecting it to godugdha Bhavana9.
  • Preparation of Mukta bhasma will be done by subjecting shodhita mukta to 3 laghuputa10.
  • Preparation of Pravala bhasma will be done by subjecting shodhita pravala to 3 gajaputa11.
  • Preparation of Shukti bhasma12, Varatika bhasma13, Shankha bhasma14 will be done by subjecting shodhita Shankha, Shukti, Varatika to puta.
  • Kamadugha Rasa is prepared by triturating equal quantities of Mukta bhasma, Praval bhasma, Mukta Shukti bhasma, Varatiika bhasma, Shankha bhasma, Gairika and Guduchi Satva in the khalva yantra1.

Analytical Study

Physical tests: i) Organoleptic

ii)pH

iii) Ash value

iv)Acid insoluble ash

v) Loss on drying

vi)Loss on ignition

Chemical tests: i) Qualitative analysis by X-RD, XRF, FTIR.

ii)) Quantitative analysis by volumetric method

Experimental study:

Animal- Wister strainalbino rats

Drug - Kamadugha Rasa

Method- Indomethacin (20mg/kg) induced ulcer model15

Method:

The albino rats of either sex are randomly distributed into 3 groups of 6 animals each.

Group- I: Serves as control group which receives Gum acacia.

Group-II: Serves as standard group which receives Ranitidine Hydrochloride

Group-III:Serves as trial group which receives Kamadugha Rasa.

The control drug, standard drug and trial drug will be given once a day for 6days, orally

to the albino rats of respective group. On 6th day night all the animals will be fasted

overnight. Next day the control drug, standard drug and trialdrug will be given 2hour

prior to the administration of ulcerogen i.e. Indomethacin orally. After 3 hours, animals

will be sacrificed by ether anaesthesia.Then the abdomen will be opened. Stomach is

dissected and screened for gastric lesions and ulcers.

Gastric lesions will be studied under compound microscope or with the help of hand lens

(10x). Ulcer index is calculated16.

Statistical analysis : Will be done by unpaired ‘t’ test

Mean ± standard deviation and diagrammatic representation will be utilized.

7.3 Does the study require any investigations or interventions to be conducted on patients

Or other humans or animals?If so please describe briefly

YES

The present study is carried out on albino rats.The albino rats of either sex with 150-200gm are taken.The animals utilized for experimental study will be sacrificed for the purpose of study. The experiments are conducted under the guidance and supervision of Guide and Co-guide.

7.4 Has ethical clearance from your institution in case of 7.3

YES

The ethical clearance has been obtained from The Institutional Animal Ethics Committee

(IAEC), Vijayanagara Institute of Medical Sciences, Bellary. Xerox copy of Ethical

Clearance certificate has been enclosed.

  1. LIST OF REFERENCES:

1. Vaidya Pandit Hariprapannaji, Rasayogasagar, Vol-I, 7th edition, Varanasi, Krishnadas

Academy Publication, 1999, verses711-713 , 260pp.

2. Annonymous,Rasatantrasara Va Siddhaprayoga Samgraha, Vol-I, 17thedition, Ajmir,

Krishna Gopal Ayurveda Bhavan Publications, 2006, 446pp.

3. Harsha Mohan, A text Book of Pathology, 5th edition, New Delhi, Jaypee Brothers

Medical publications, Reprint 2006, 18th chapter, 563pp.

4. Shri Sadananda Sharma, Rasatarangini, edited by Kashinath Shastri, 11th Edition, New

Delhi, Motilal Banarasidas Publication, 1979,23rd Taranga, verse 68, 613pp.

5. ibid,verse 132, 627pp.

6. ibid 12th Taranga, verse 66, 296pp.

7. ibid, verse 89, 300pp.

8. Shri Shankara, Rasachandamshu, Purvakhanda, Kannada translation by Dr. A. Halasikar,

Hubli, “Atreyasadana”,1850,verse 321 , 84pp.

9. Shri Vagbhatacharya, Rasa Ratna Samuchhaya, Vol I, edited by Dattatreya Ananta

Kulkarni, New Delhi,Meharchanda Lachamanadas Publication, Reprint 1998, 3rd

chapter,verse 49 , 49pp.

10. Shri Sadananda Sharma, Rasatarangini, edited by Kashinath Shastri, 11th edition, New

Delhi, Motilal Banarasidas Publication, 1979, 23rd Taranga,verse 70 , 613pp.

11. ibid, verse 135, 628pp.

12. ibid 12th Taranga, verse 67, 296pp.

13. ibid, verses 91-93, 301pp.

14. ibid,verses 17- 19, 288pp.

15. Laurence D.R., Evaluation of Drugs and Pharmacomatrics, 6th edition, London,

Academy Press Inc Ltd. Publications,1964, 158pp,161pp.

16. S.K.Kulkarni, Hand Book of Experimental Pharmacology, 3rd edition, (Pitampura) Delhi,

Vallabh Prakashan Publication, Reprint 2006, Chapter 4, 149pp.

  1. SIGNATURE OF CANDIDATE

10. REMARKS OF GUIDE

11. NAME AND DESIGNATION ( IN BLOCK LETTERS )

11.1 GUIDE: Dr.SUREKHA.S. MEDIKERI M D(AYU)

ASSISTANT PROFESSOR ,

DEPARTMENT OF P.G. STUDIES IN RASASHASTRA,

TARANATH GOVT AYURVEDIC MEDICAL COLLEGE

BELLARY-583101

11.2 SIGNATURE:

11.3 CO-GUIDE: Dr.MURGHESH.J.V M.D.

ASSISTANT PROFESSOR

DEPARTMENT OF PHARMACOLOGY

VIJAYANAGAR INSTITUTE OF MEDICAL SCIENCES,

BELLARY-583101.

11.4 SIGNATURE:

11.5 HEAD OF THE DEPARTMENT

Dr.M.S.DODDAMANI M D(AYU)

PROFESSOR AND HEAD OF THE DEPARTMENT,

DEPARTMENT OF P.G.STUDIES IN RASASHASTRA,

TARANATH GOVT AYURVEDIC MEDICAL COLLEGE

BELLARY-583101

11.6 SIGNATURE

12.

12.1 REMARKS OF CHAIRMAN AND PRINCIPAL:

12.2 SIGNATURE