Data S1. MAGI Proteins and Their Binding Partners. All Three MAGI Family Members Have

Data S1. MAGI Proteins and Their Binding Partners. All Three MAGI Family Members Have

Data S1. MAGI proteins and their binding partners. All three MAGI family members have the same domain structure, shown in the yellow column on the left. The boxes to the right of this show, for each of the MAGIs, which proteins bind to which of the MAGI domains. Boxes spanning more than one domain correspond to proteins whose binding site in MAGI has not been determined precisely but is known to lie somewhere within the indicated region.

(synonyms BAIAP1, BAP1, AIP3, WWP3, TNRC19) / MAGI-2
(synonyms AIP1, ACVRIP1, Acvrinp-1, ARIP1, S-SCAM) / MAGI-3
(synonym SLIPR)
Domain / Binding partner(s)
PDZ0 / RapGEP [1]
GuK / SAPAP [2]
WW1 / atrophin-1 [3] / atrophin-1 [3]
WW2 / synaptopodin [4] / SMAD3 [5]
PDZ1 / ß1-adrenergic receptor [6]
mNET-1 [7]
HPV16/18 E6 protein [8, 9],
adenovirus-9 E4 protein [10]
JAM4 [11] / MuSK [12]
ASIC3 [13] / ß1-adrenergic receptor [6]
neuroligin [2]
GDP-GTP exchange factor nRAP-GEP [14]
HPV16/18 E6 protein [8] / KIF1B [15] / HPV16/18 E6 protein [8]
Ltap [16]
Frizzled-4 [16]
Frizzled-7 [16]
PDZ2 / PTEN [17-20] / RPTPß [21] / PTEN [22] / RPTPß [21]
PDZ3 / adenovirus-9 E4 protein [10]
PDZ4 / BAI-1 [23]
JAM4 [11]
Delta1/DeltaD/DeltaC [this paper] / Delta1 [32]
Delta1/DeltaD/DeltaC [this paper] / MAGUIN-1 [24] / Delta1/DeltaD/
DeltaC [this paper]
BAI-1 [22]
PDZ5 / alpha-actinin-4 [4],
megalin [25],
ß-catenin [26] / delta2 glutamate receptor [27]
activin receptor IIA [5]
tamalin [28]
NMDA receptors [2, 29]
ß-catenin [30]
delta-catenin/NPRAP [31] / NMDA receptor 2B [22]


[1] Mino et al. (2000).

[2] Hirao et al. (1998).

[3] Wood et al. (1998).

[4] Patrie et al. (2002).

[5] Shoji et al. (2000).

[6] Xu et al. (2001).

[7] Dobrosotskaya (2001).

[8] Thomas et al. (2002).

[9] Thomas et al. (2001).

[10] Glaunsinger et al. (2000).

[11] Hirabayashi et al. (2003).

[12] Strochlic et al. (2001).

[13] Hruska-Hageman et al. (2004).

[14] Ohtsuka et al. (1999).

[15] Mok et al. (2002).

[16] Yao et al. (2004).

[17] Wu et al. (2000a).

[18] Vazquez et al. (2001).

[19] Torres et al. (2003).

[20] Tolkacheva et al. (2001).

[21] Adamsky et al. (2003).

[22] Wu et al. (2000b).

[23] Shiratsuchi et al. (1998).

[24] Yao et al. (1999).

[25] Patrie et al. (2001).

[26] Dobrosotskaya and James (2000).

[27] Yap et al. (2003).

[28] Kitano et al. (2003).

[29] Hirao et al. (2000a; 2000b).

[30] Nishimura et al. (2002).

[31] Ide et al. (1999).

[32] Pfister et al. (2003)



Adamsky, K., Arnold, K., Sabanay, H. and Peles, E. (2003). Junctional protein MAGI-3 interacts with receptor tyrosine phosphatase beta (RPTPbeta) and tyrosine-phosphorylated proteins. J. Cell Sci.116, 1279-1289.

Dobrosotskaya, I. Y. (2001). Identification of mNET1 as a candidate ligand for the first PDZ domain of MAGI-1. Biochem. Biophys. Res. Commun.283, 969-975.

Dobrosotskaya, I. Y. and James, G. L. (2000). MAGI-1 interacts with beta-catenin and is associated with cell-cell adhesion structures. Biochem. Biophys. Res. Commun.270, 903-909.

Glaunsinger, B. A., Lee, S. S., Thomas, M., Banks, L. and Javier, R. (2000). Interactions of the PDZ-protein MAGI-1 with adenovirus E4-ORF1 and high-risk papillomavirus E6 oncoproteins. Oncogene19, 5270-5280.

Hirabayashi, S., Tajima, M., Yao, I., Nishimura, W., Mori, H. and Hata, Y. (2003). JAM4, a junctional cell adhesion molecule interacting with a tight junction protein, MAGI-1. Mol. Cell. Biol.23, 4267-4282.

Hirao, K., Hata, Y., Deguchi, M., Yao, I., Ogura, M., Rokukawa, C., Kawabe, H., Mizoguchi, A. and Takai, Y. (2000a). Association of synapse-associated protein 90/ postsynaptic density-95-associated protein (SAPAP) with neurofilaments. Genes Cells5, 203-210.

Hirao, K., Hata, Y., Ide, N., Takeuchi, M., Irie, M., Yao, I., Deguchi, M., Toyoda, A., Sudhof, T. C. and Takai, Y. (1998). A novel multiple PDZ domain-containing molecule interacting with N-methyl-D-aspartate receptors and neuronal cell adhesion proteins. J. Biol. Chem.273, 21105-21110.

Hirao, K., Hata, Y., Yao, I., Deguchi, M., Kawabe, H., Mizoguchi, A. and Takai, Y. (2000b). Three isoforms of synaptic scaffolding molecule and their characterization. Multimerization between the isoforms and their interaction with N-methyl-D-aspartate receptors and SAP90/PSD-95-associated protein. J. Biol. Chem.275, 2966-2972.

Hruska-Hageman, A. M., Benson, C. J., Leonard, A. S., Price, M. P. and Welsh, M. J. (2004). PSD-95 and Lin-7b interact with ASIC3 and have opposite effects on H+-gated current. J. Biol. Chem. (in press).

Ide, N., Hata, Y., Deguchi, M., Hirao, K., Yao, I. and Takai, Y. (1999). Interaction of S-SCAM with neural plakophilin-related Armadillo-repeat protein/delta-catenin. Biochem. Biophys. Res. Commun.256, 456-461.

Kitano, J., Yamazaki, Y., Kimura, K., Masukado, T., Nakajima, Y. and Nakanishi, S. (2003). Tamalin is a scaffold protein that interacts with multiple neuronal proteins in distinct modes of protein-protein association. J. Biol. Chem.278, 14762-14768.

Mino, A., Ohtsuka, T., Inoue, E. and Takai, Y. (2000). Membrane-associated guanylate kinase with inverted orientation (MAGI)-1/brain angiogenesis inhibitor 1-associated protein (BAP1) as a scaffolding molecule for Rap small G protein GDP/GTP exchange protein at tight junctions. Genes Cells5, 1009-1016.

Mok, H., Shin, H., Kim, S., Lee, J. R., Yoon, J. and Kim, E. (2002). Association of the kinesin superfamily motor protein KIF1Balpha with postsynaptic density-95 (PSD-95), synapse-associated protein-97, and synaptic scaffolding molecule PSD-95/discs large/zona occludens-1 proteins. J. Neurosci.22, 5253-5258.

Nishimura, W., Yao, I., Iida, J., Tanaka, N. and Hata, Y. (2002). Interaction of synaptic scaffolding molecule and Beta -catenin. J. Neurosci.22, 757-765.

Ohtsuka, T., Hata, Y., Ide, N., Yasuda, T., Inoue, E., Inoue, T., Mizoguchi, A. and Takai, Y. (1999). nRap GEP: a novel neural GDP/GTP exchange protein for rap1 small G protein that interacts with synaptic scaffolding molecule (S-SCAM). Biochem. Biophys. Res. Commun.265, 38-44.

Patrie, K. M., Drescher, A. J., Goyal, M., Wiggins, R. C. and Margolis, B. (2001). The membrane-associated guanylate kinase protein MAGI-1 binds megalin and is present in glomerular podocytes. J. Am. Soc. Nephrol.12, 667-677.

Patrie, K. M., Drescher, A. J., Welihinda, A., Mundel, P. and Margolis, B. (2002). Interaction of two actin-binding proteins, synaptopodin and alpha-actinin-4, with the tight junction protein MAGI-1. J. Biol. Chem.277, 30183-30190.

Pfister, S., Przemeck, G. K., Gerber, J. K., Beckers, J., Adamski, J. and de Angelis, M. H. (2003). Interaction of the MAGUK family member Acvrinp1 and the cytoplasmic domain of the Notch ligand Delta1. J. Mol. Biol.333, 229-235.

Shiratsuchi, T., Futamura, M., Oda, K., Nishimori, H., Nakamura, Y. and Tokino, T. (1998). Cloning and characterization of BAI-associated protein 1: a PDZ domain-containing protein that interacts with BAI1. Biochem. Biophys. Res. Commun.247, 597-604.

Shoji, H., Tsuchida, K., Kishi, H., Yamakawa, N., Matsuzaki, T., Liu, Z., Nakamura, T. and Sugino, H. (2000). Identification and characterization of a PDZ protein that interacts with activin type II receptors. J. Biol. Chem.275, 5485-5492.

Strochlic, L., Cartaud, A., Labas, V., Hoch, W., Rossier, J. and Cartaud, J. (2001). MAGI-1c: a synaptic MAGUK interacting with muSK at the vertebrate neuromuscular junction. J. Cell Biol.153, 1127-1132.

Thomas, M., Glaunsinger, B., Pim, D., Javier, R. and Banks, L. (2001). HPV E6 and MAGUK protein interactions: determination of the molecular basis for specific protein recognition and degradation. Oncogene20, 5431-5439.

Thomas, M., Laura, R., Hepner, K., Guccione, E., Sawyers, C., Lasky, L. and Banks, L. (2002). Oncogenic human papillomavirus E6 proteins target the MAGI-2 and MAGI-3 proteins for degradation. Oncogene21, 5088-5096.

Tolkacheva, T., Boddapati, M., Sanfiz, A., Tsuchida, K., Kimmelman, A. C. and Chan, A. M. (2001). Regulation of PTEN binding to MAGI-2 by two putative phosphorylation sites at threonine 382 and 383. Cancer Res.61, 4985-4989.

Torres, J., Rodriguez, J., Myers, M. P., Valiente, M., Graves, J. D., Tonks, N. K. and Pulido, R. (2003). Phosphorylation-regulated cleavage of the tumor suppressor PTEN by caspase-3: implications for the control of protein stability and PTEN-protein interactions. J. Biol. Chem.

Vazquez, F., Grossman, S. R., Takahashi, Y., Rokas, M. V., Nakamura, N. and Sellers, W. R. (2001). Phosphorylation of the PTEN tail acts as an inhibitory switch by preventing its recruitment into a protein complex. J. Biol. Chem.276, 48627-48630.

Wood, J. D., Yuan, J., Margolis, R. L., Colomer, V., Duan, K., Kushi, J., Kaminsky, Z., Kleiderlein, J. J., Sharp, A. H. and Ross, C. A. (1998). Atrophin-1, the DRPLA gene product, interacts with two families of WW domain-containing proteins. Mol. Cell. Neurosci.11, 149-160.

Wu, X., Hepner, K., Castelino-Prabhu, S., Do, D., Kaye, M. B., Yuan, X. J., Wood, J., Ross, C., Sawyers, C. L. and Whang, Y. E. (2000a). Evidence for regulation of the PTEN tumor suppressor by a membrane-localized multi-PDZ domain containing scaffold protein MAGI-2. Proc. Natl. Acad. Sci. USA97, 4233-4238.

Wu, Y., Dowbenko, D., Spencer, S., Laura, R., Lee, J., Gu, Q. and Lasky, L. A. (2000b). Interaction of the tumor suppressor PTEN/MMAC with a PDZ domain of MAGI3, a novel membrane-associated guanylate kinase. J. Biol. Chem.275, 21477-21485.

Xu, J., Paquet, M., Lau, A. G., Wood, J. D., Ross, C. A. and Hall, R. A. (2001). beta 1-adrenergic receptor association with the synaptic scaffolding protein membrane-associated guanylate kinase inverted-2 (MAGI-2). Differential regulation of receptor internalization by MAGI-2 and PSD-95. J. Biol. Chem.276, 41310-41317.

Yao, I., Hata, Y., Ide, N., Hirao, K., Deguchi, M., Nishioka, H., Mizoguchi, A. and Takai, Y. (1999). MAGUIN, a novel neuronal membrane-associated guanylate kinase-interacting protein. J. Biol. Chem.274, 11889-11896.

Yao, R., Natsume, Y. and Noda, T. (2004). MAGI-3 is involved in the regulation of the JNK signaling pathway as a scaffold protein for frizzled and Ltap. Oncogene23, 6023-6030.

Yap, C. C., Muto, Y., Kishida, H., Hashikawa, T. and Yano, R. (2003). PKC regulates the delta2 glutamate receptor interaction with S-SCAM/MAGI-2 protein. Biochem. Biophys. Res. Commun.301, 1122-1128.