A Proteomic Analysis of Seeds from Bt-Transgenic Brassica Napus and Hybrids with Wild B. Juncea

A proteomic analysis of seeds from Bt-transgenic Brassica napus and hybrids with wild B. juncea

Yongbo Liu1,†,*, Ying-Xue Zhang2,4,†, Song-Quan Song2, Junsheng Li1, C. Neal Stewart, Jr5, Wei Wei3, Yujie Zhao1, Wei-Qing Wang2,*

1 State Key Laboratory of Environmental Criteria and Risk Assessment, ChineseResearchAcademy of Environmental Sciences, 8 Dayangfang, Beijing 100012, China

2Key Laboratory of Plant Resources and BeijingBotanical Garden, Institute of Botany, ChineseAcademy of Sciences, Beijing 100093, China

3State Key Laboratory of Vegetation and Environmental Change, Institute of Botany, ChineseAcademy of Sciences, Beijing 100093, China

4College of Chemistry and Chemical Engineering, HenanUniversity, Kaifeng, 475001, China

5Department of Plant Sciences, University of Tennessee, 2431 Joe Johnson Drive, Knoxville, TN37996-4561, USA

†Contribution equally

*Author for Correspondence:

Emails: (Y.L.); (W.W.).

Tel/Fax: +86 10 84910906 (Y.L.); +86 10 62836049(W.W.).

Supplementary information

Figure S1.ELISA detection ofCry1Ac protein in transgenic and non-transgenic seeds. Columns from left to right were non-transgenic Brassica napus (BN), wild B. juncea (BJ), non-transgenic hybrids (BJBN) from BJ with BN, transgenic B. napus (tBN) and transgenic hybrids from BJ with tBN (BJtBN), respectively. Blue color indicates the expression of Cry1Ac proteins.