A, Orfs Were Numbered Consecutively
Supplementary Table 1. Putativeopen reading frames (ORFs) identified in theRS138 genome obtainedby ORF Finder search.
ORFa / Start / End / Strand / Putative functionb / size (aa) / Closest relativec / Alignment region (aa)d / %Identity / E-valuee / Sourcef / Sequence IDg
1 / 15 / 161 / - / hypothetical protein / 48
2 / 180 / 791 / - / hypothetical protein / 203 / hypothetical protein / 1-202/205 / 85 / 6e122 / Ralstonia solanacearum / WP_042590051.1
3 / 1105 / 1533 / - / hypothetical protein / 142 / hypothetical protein / 19-142/135 / 50 / 2e27 / Ralstonia solanacearum / WP_042590050.1
4 / 1721 / 2065 / + / transcriptional regulator / 114 / putative transcriptional regulator / 13-114/103 / 75 / 1e47 / R. solanacearum K60-1 / CCF96499.1
5 / 2091 / 2651 / + / hypothetical protein / 186 / hypothetical protein / 1-163/178 / 73 / 3e79 / Ralstonia solanacearum / WP_042590049.1
6 / 2593 / 3666 / + / integrase / 357 / integrase / 1-258/748 / 82 / 2e136 / Ralstonia solanacearum / WP_042590174.1
7 / 3425 / 4840 / + / integrase / 471 / integrase / 1-471/748 / 94 / 0.0 / Ralstonia solanacearum / WP_042590174.1
8 / 4857 / 5189 / + / phage DNA transposition protein / 110 / putative phage DNA transposition protein / 1-63/254 / 78 / 1e23 / R. solanacearum K60-1 / CCF96502.1
9 / 5126 / 5620 / + / transposase / 164 / transposase / 1-164/254 / 95 / 7e109 / Ralstonia solanacearum / WP_042590175.1
10 / 5617 / 5895 / + / hypothetical protein / 92 / hypothetical protein / 11-92/91 / 90 / 2e53 / Ralstonia solanacearum / WP_042590176.1
11 / 6205 / 6525 / + / hypothetical protein / 106 / hypothetical protein / 1-106/213 / 83 / 1e56 / Ralstonia solanacearum / WP_042590177.1
12 / 6515 / 6973 / + / hypothetical protein / 152 / hypothetical protein / 8-152/146 / 92 / 2e91 / Ralstonia solanacearum / WP_042590178.1
13 / 6829 / 7332 / + / hypothetical protein / 167 / hypothetical protein / 79-167/113 / 79 / 8e54 / Ralstonia solanacearum / WP_042590179.1
14 / 7332 / 7481 / + / hypothetical protein / 49 / hypothetical protein / 1-48/51 / 63 / 1e12 / R. solanacearum K60-1 / CCF96507.1
15 / 7492 / 7830 / + / hypothetical protein / 112 / hypothetical protein / 1-79/155 / 94 / 3e44 / Ralstonia solanacearum / WP_042590180.1
16 / 7955 / 8539 / + / hypothetical protein / 194 / hypothetical protein / 1-194/194 / 87 / 5e119 / Ralstonia solanacearum / WP_042590181.1
17 / 8539 / 8886 / + / hypothetical protein / 115 / hypothetical protein / 1-115/115 / 78 / 2e54 / Ralstonia solanacearum / WP_042590182.1
18 / 9016 / 9522 / + / sulfatetransporter / 168 / sulfatetransporter / 1-168/207 / 92 / 9e108 / Ralstonia solanacearum / WP_042590183.1
19 / 9532 / 9969 / + / hypothetical protein / 145 / hypothetical protein / 52-145/135 / 81 / 3e48 / Ralstonia solanacearum / WP_042590048.1
20 / 10064 / 10198 / + / hypothetical protein / 44
21 / 10202 / 10546 / + / hypothetical protein / 114 / hypothetical protein / 1-34/145 / 57 / 0.006 / Ralstonia solanacearum / WP_042590047.1
22 / 10747 / 10881 / + / hypothetical protein / 44
23 / 10885 / 11322 / + / hypothetical protein / 145 / hypothetical protein / 1-145/145 / 83 / 3e87 / Ralstonia solanacearum / WP_042590047.1
24 / 11325 / 11717 / + / hypothetical protein / 130 / hypothetical protein / 1-130/130 / 92 / 3e80 / Ralstonia solanacearum / WP_042590046.1
25 / 11721 / 12164 / + / mor transcription / 147 / hypothetical protein / 1-147/147 / 93 / 2e92 / Ralstonia solanacearum / WP_042590045.1
26 / 12315 / 12755 / + / virion protein / 146 / hypothetical protein / 1-145/ 145 / 89 / 2e89 / Ralstonia solanacearum / WP_042590044.1
27 / 12752 / 12997 / + / hypothetical protein / 81 / hypothetical protein / 1-81/81 / 95 / 9e-48 / Ralstonia solanacearum / WP_042590043.1
28 / 12990 / 13586 / + / lysis protein / 198 / lysis protein / 1-194/205 / 39 / 1e23 / Pseudomonas aeruginosa / WP_034065874.1
29 / 13576 / 13803 / + / hypothetical protein / 75 / hypothetical protein / 7-75/69 / 84 / 5e33 / Ralstonia solanacearum / WP_042592080.1
30 / 13804 / 14025 / + / hypothetical protein / 73 / hypothetical protein / 1-73/73 / 96 / 7e32 / Ralstonia solanacearum / WP_042590042.1
31 / 14025 / 14525 / + / DNAbinding
protein / 166 / DNAbindingprotein / 1-166/166 / 96 / 8e109 / Ralstonia solanacearum / WP_042590041.1
32 / 14518 / 16296 / + / hypothetical protein / 592 / hypothetical protein / 1-592/592 / 93 / 0.0 / Ralstonia solanacearum / WP_042590040.1
33 / 16302 / 17912 / + / hypothetical protein / 536 / hypothetical protein / 1-534/534 / 92 / 0.0 / Ralstonia solanacearum / WP_042590039.1
34 / 17958 / 19334 / + / phage Mu
protein / 458 / hypothetical protein / 1-350/446 / 82 / 0.0 / Ralstonia solanacearum / WP_042590038.1
35 / 19291 / 19758 / + / virion morphogenesis protein / 155 / hypothetical protein / 7-153/147 / 86 / 2e-88 / Ralstonia solanacearum / WP_042592079.1
36 / 19967 / 20242 / + / hypothetical protein / 91 / hypothetical protein / 6-90/159 / 68 / 9e33 / Acidithiobacillus
thiooxidans / WP_031571147.1
37 / 20570 / 21622 / + / Mu-like
prophageI
protein / 350 / hypothetical protein / 1-350/348 / 88 / 0.0 / Ralstonia solanacearum / WP_042590037.1
38 / 21667 / 22074 / + / bacteriophage protein / 135 / bacteriophage protein / 1-135/135 / 87 / 1e75 / Ralstonia solanacearum / WP_042590036.1
39 / 22115 / 23008 / + / Mu-like
prophagemajor
headsubunitgpT / 297 / phageheadprotein / 1-297/297 / 89 / 0.0 / Ralstonia solanacearum / WP_042590035.1
40 / 23126 / 23560 / + / hypothetical protein / 144 / hypothetical protein / 1-144/146 / 75 / 2e-56 / Ralstonia solanacearum / WP_042590034.1
41 / 23567 / 23983 / + / hypothetical protein / 138 / hypothetical protein / 1-138/138 / 89 / 6e78 / Ralstonia solanacearum / WP_042590033.1
42 / 24133 / 24456 / + / hypothetical protein / 107 / hypothetical protein / 1-105/156 / 83 / 2e56 / Ralstonia solanacearum / WP_042590032.1
43 / 24432 / 24623 / + / hypothetical protein / 63 / hypothetical protein / 1-63/61 / 83 / 1e27 / Ralstonia solanacearum / WP_042590031.1
44 / 24663 / 25412 / + / hypothetical protein / 249 / hypothetical protein / 1-249/250 / 89 / 3e162 / Ralstonia solanacearum / WP_042590030.1
45 / 25434 / 25790 / + / hypothetical protein / 118 / hypothetical protein / 1-118/118 / 92 / 1e69 / Ralstonia solanacearum / WP_042590029.1
46 / 25937 / 30634 / + / tape measure protein / 1565 / hypothetical protein / 1-1565/1565 / 95 / 0.0 / Ralstonia solanacearum / WP_042590028.1
47 / 30631 / 31035 / + / hypothetical protein / 134 / hypothetical protein / 1-134/134 / 93 / 1e86 / Ralstonia solanacearum / WP_042590027.1
48 / 31032 / 34673 / + / membraneprotein / 1213 / membraneprotein / 1-1213/1213 / 91 / 0.0 / Ralstonia solanacearum / WP_042590026.1
49 / 34689 / 35846 / + / hypothetical protein / 385 / hypothetical protein / 1-210/210 / 89 / 4e121 / Ralstonia solanacearum / WP_042592078.1
50 / 35857 / 37587 / + / hypothetical protein / 576 / hypothetical protein / 9-572/909 / 36 / 2e-78 / Cupriaiusnecator / WP_042886294.1
51 / 37584 / 37982 / + / hypothetical protein / 132 / hypothetical protein / 1-132/133 / 89 / 7e65 / Ralstonia solanacearum / WP_042590025.1
52 / 37986 / 39866 / + / hypothetical protein / 626 / hypothetical protein / 1-625/626 / 85 / 0.0 / Ralstonia solanacearum / WP_042590024.1
53 / 39882 / 40106 / + / hypothetical protein / 74 / hypothetical protein / 1-74/74 / 96 / 3e42 / Ralstonia solanacearum / WP_042592076.1
54 / 40312 / 40773 / + / hypothetical protein / 153 / hypothetical protein / 1-153/151 / 92 / 3e99 / Ralstonia solanacearum / WP_043899403.1
55 / 41077 / 41463 / + / Esterases-lipase / 128 / hypothetical protein / 1-128/229 / 84 / 7e-71 / Ralstonia
mannitolilytica / WP_045785547.1
56 / 41460 / 41825 / + / hypothetical protein / 121 / hypothetical protein / 1-112/118 / 88 / 3e45 / Ralstonia solanacearum / WP_0425052.1
a, ORFs were numbered consecutively.
b, Predicted function is based on amino acid sequence identity.
c, The most closely related gene.
d, Alignment region (aa) of RS138 ORF / size (aa) of most closely related ORF
e, BLASTP values were generated by BLAST analysis.
f, Source of the most closely related gene.
g, Sequence ID of the most closely related gene.