Table S1 Phenotypic Performance of the Rils and Their Parents Under Hydroponic Culture

Electronic Supplementary Materials

Table S1 Nutrient solution ingredients for wheat seedling growth

Table S2Phenotypic performance of the RILs and their parents under hydroponic culture, pot and field trials

Table S3Analysis of variance for investigated traits under hydroponic culture, pot and field trials

TableS4Spearmancorrelation coefficients (r) between investigated traits

Table S5Additive QTLs detected in five K treatments under hydroponic culture, pot trial and field trial.

Table S1 Nutrient solution ingredients for wheat seedling growth

Ingredients / Concentration
(mmol/L) / Ingredients / Concentration
(mol/L)
(NH4)2SO4·H2O / 1.0 / H3BO3 / 1.0
Ca(NO3)2·4H2O / 1.0 / CuSO4·5H2O / 0.5
KH2PO4 / 0.2 / ZnSO4·7H2O / 1.0
KCl / 1.8 / MnSO4·H2O / 1.0
MgSO4·7H2O / 0.5 / FeEDTA / 100
CaCl2 / 1.5 / (NH4)6Mo7O24·4H2O / 0.1

Table S2Phenotypic performance of the RILs and their parents under hydroponic culture, pot and field trials

Traita / Treatment / Parentsb / RIL population
Ch / Sh / Averagec / SDd / CV (%)e / Max / Min / hB2(%)f
Hydroponic culture
RDW / HK / 18.56 / 28.44 / 21.88 C / 4.42 / 20.21 / 40.22 / 13.67 / 98.3
(mg·plant-1) / MK / 28.89 / 26.22 / 22.73 A / 4.07 / 17.91 / 37.56 / 14.89
LK1 / 24.22 / 23.67 / 22.45 AB / 4.53 / 20.18 / 36.89 / 12.67
LK2 / 22.44 / 32.00 / 22.57 A / 4.60 / 20.38 / 36 / 14.33
LK3 / 22.56 / 23.89 / 22.02 BC / 4.06 / 18.43 / 36.38 / 13
SDW / HK / 71.67 / 99.33 / 79.06 B / 14.14 / 17.89 / 123.89 / 52 / 95.9
(mg·plant-1) / MK / 100.11 / 90.78 / 81.63 A / 14.33 / 17.55 / 135.89 / 51.44
LK1 / 94.11 / 84.00 / 77.98 C / 13.83 / 17.73 / 117.22 / 42
LK2 / 72.00 / 101.78 / 70.56 D / 13.36 / 18.94 / 107.56 / 41.78
LK3 / 55.89 / 63.67 / 59.66 E / 10.11 / 16.94 / 106.25 / 39.33
RSDW / HK / 0.26 / 0.29 / 0.28 D / 0.03 / 9.72 / 0.35 / 0.21 / 90.0
MK / 0.29 / 0.29 / 0.28 D / 0.03 / 8.98 / 0.35 / 0.23
LK1 / 0.26 / 0.28 / 0.29 CD / 0.02 / 8.65 / 0.35 / 0.22
LK2 / 0.31 / 0.31 / 0.32 BC / 0.03 / 9.51 / 0.41 / 0.26
LK3 / 0.40 / 0.38 / 0.37 A / 0.04 / 9.71 / 0.47 / 0.3
TDW / HK / 90.22 / 127.78 / 100.94 BC / 18.15 / 17.98 / 164.11 / 68.89 / 96.8
(mg·plant-1) / MK / 129.00 / 117.00 / 104.36 A / 18.02 / 17.27 / 173.44 / 67.89
LK1 / 118.33 / 107.67 / 100.44 C / 18.01 / 17.94 / 154.11 / 55.89
LK2 / 94.44 / 133.78 / 93.12 D / 17.57 / 18.87 / 143.56 / 57.89
LK3 / 78.44 / 87.56 / 81.68 E / 13.71 / 16.79 / 142.63 / 52.33
RKC / HK / 0.73 / 1.17 / 0.93 A / 0.21 / 22.90 / 1.6 / 0.54 / 62.9
(mg·plant-1) / MK / 1.08 / 1.01 / 0.88 A / 0.20 / 22.60 / 1.8 / 0.45
LK1 / 0.54 / 0.50 / 0.57 B / 0.14 / 23.89 / 0.96 / 0.22
LK2 / 0.17 / 0.21 / 0.17 C / 0.06 / 35.48 / 0.36 / 0.06
LK3 / 0.05 / 0.09 / 0.11 C / 0.05 / 41.48 / 0.29 / 0.03
SKC / HK / 3.50 / 5.06 / 4.27 A / 0.72 / 16.97 / 6.11 / 2.36 / 68.0
(mg·plant-1) / MK / 4.83 / 4.01 / 3.84 B / 0.76 / 19.79 / 7.5 / 2.27
LK1 / 2.98 / 2.86 / 2.51 C / 0.47 / 18.87 / 3.67 / 1.3
LK2 / 1.66 / 2.70 / 1.71 D / 0.33 / 19.45 / 2.84 / 0.9
LK3 / 0.94 / 1.12 / 1.02 E / 0.18 / 18.02 / 1.8 / 0.45
RSKC / HK / 0.21 / 0.23 / 0.22 A / 0.04 / 16.95 / 0.34 / 0.14 / 73.8
MK / 0.22 / 0.25 / 0.23 A / 0.05 / 19.83 / 0.48 / 0.15
LK1 / 0.18 / 0.18 / 0.23 A / 0.03 / 14.36 / 0.29 / 0.14
LK2 / 0.10 / 0.08 / 0.096 B / 0.03 / 27.14 / 0.16 / 0.05
LK3 / 0.05 / 0.08 / 0.11 B / 0.04 / 34.74 / 0.28 / 0.04
TKC / HK / 4.23 / 6.23 / 5.20 A / 0.89 / 17.07 / 7.65 / 2.99 / 65.6
(mg·plant-1) / MK / 5.90 / 5.02 / 4.72 B / 0.89 / 18.89 / 8.69 / 2.94
LK1 / 3.52 / 3.36 / 3.08 C / 0.59 / 19.11 / 4.51 / 1.61
LK2 / 1.83 / 2.91 / 1.88 D / 0.37 / 19.89 / 3.21 / 1.02
LK3 / 0.99 / 1.21 / 1.13 E / 0.21 / 18.97 / 2.09 / 0.51
RKUE / HK / 0.47 / 0.69 / 0.52 E / 0.11 / 21.92 / 1.01 / 0.31 / 43.3
(mg2RDW·ug-1RKC) / MK / 0.78 / 0.68 / 0.59 D / 0.12 / 20.42 / 1.06 / 0.33
LK1 / 1.09 / 1.12 / 0.90 C / 0.21 / 22.83 / 1.53 / 0.53
LK2 / 3.00 / 4.77 / 3.22 B / 0.77 / 23.99 / 6.33 / 1.87
LK3 / 10.09 / 6.66 / 4.68 A / 1.46 / 31.14 / 9.49 / 1.71
SKUE / HK / 1.47 / 1.95 / 1.47 E / 0.31 / 20.98 / 2.53 / 0.93 / 82.7
(mg2SDW·ug-1SKC) / MK / 2.08 / 2.06 / 1.75 D / 0.37 / 21.08 / 3.02 / 1.01
LK1 / 2.97 / 2.47 / 2.42 C / 0.44 / 18.22 / 3.82 / 1.35
LK2 / 3.13 / 3.84 / 2.92 B / 0.60 / 20.45 / 4.62 / 1.74
LK3 / 3.34 / 3.61 / 3.52 A / 0.67 / 19.10 / 6.27 / 2.12
TKUE / HK / 1.92 / 2.62 / 1.97 E / 0.41 / 20.74 / 3.52 / 1.24 / 73.3
(mg2TDW·ug-1TKC) / MK / 2.82 / 2.73 / 2.32 D / 0.47 / 20.12 / 3.78 / 1.40
LK1 / 3.98 / 3.45 / 3.28 C / 0.60 / 18.43 / 5.28 / 1.94
LK2 / 4.88 / 6.14 / 4.63 B / 0.92 / 19.78 / 7.20 / 3.05
LK3 / 6.24 / 6.34 / 5.92 A / 1.03 / 17.42 / 9.75 / 3.75

a The abbreviations of traits refer to Table 2

b Ch, Chuan 35050; Sh, Shannong 483.

c Means in the same column followed by the same letter do not differ significantlyaccording to the LSD test (letter indicating p = 0.05).

d SD, Standard Deviation

e CV, (Coefficient of Variation,%) = SD/average × 100

fhB2 (%), broad-sense heritability

Table S2Continued

Trait / Treatment / Parents / RIL population / hB2
Ch / Sh / Average / SD / CV (%) / Max / Min / (%)
Pot tail
TGW / HK / 38.35 / 49.32 / 43.55 A / 6.15 / 14.12 / 59.83 / 27.12 / 98.5
(g) / MK / 40.15 / 47.90 / 41.89 B / 6.14 / 14.65 / 59.95 / 25.75
LK / 36.10 / 48.95 / 41.77 B / 6.18 / 14.79 / 58.90 / 26.47
GN / HK / 70.56 / 70.70 / 51.42 B / 6.32 / 12.07 / 68.33 / 34.60 / 95.0
(number) / MK / 61.78 / 55.89 / 52.41 AB / 5.74 / 11.16 / 64.80 / 33.70
LK / 55.30 / 58.00 / 46.52 C / 7.00 / 13.04 / 74.60 / 37.89
SN / HK / 4.67 / 4.50 / 5.13 AB / 0.94 / 18.31 / 9.60 / 3.33 / 93.8
(number) / MK / 3.33 / 5.00 / 5.26 A / 0.98 / 18.63 / 10.56 / 3.13
LK / 3.60 / 4.00 / 4.93 B / 1.02 / 20.63 / 9.80 / 2.78
GWP / HK / 8.21 / 10.20 / 7.50 A / 1.46 / 19.44 / 12.25 / 4.45 / 92.5
(g·plant-1) / MK / 5.37 / 8.70 / 7.28 AB / 1.54 / 21.15 / 11.75 / 3.93
LK / 4.67 / 7.38 / 7.03 B / 1.78 / 25.02 / 13.89 / 3.24
StWP / HK / 1.87 / 2.28 / 9.25 AB / 1.73 / 18.68 / 14.45 / 5.33 / 89.2
(g·plant-1) / MK / 1.51 / 2.32 / 9.27 A / 2.18 / 23.52 / 21.78 / 5.04
LK / 1.34 / 2.17 / 7.96 C / 1.91 / 23.98 / 14.07 / 4.03
GKC / HK / 37.57 / 31.40 / 26.80 AB / 8.38 / 31.03 / 64.30 / 10.48 / 83.9
(mg·plant-1) / MK / 20.56 / 31.83 / 29.07 A / 7.27 / 24.95 / 60.13 / 14.21
LK / 15.39 / 23.72 / 25.63 B / 7.13 / 27.39 / 55.63 / 8.57
StKC / HK / 31.36 / 29.93 / 158.17 A / 36.28 / 22.94 / 288.02 / 75.41 / 79.0
(mg·plant-1) / MK / 20.67 / 40.62 / 149.78 B / 42.01 / 28.12 / 374.68 / 77.18
LK / 16.28 / 29.65 / 109.18 C / 35.78 / 31.26 / 232.91 / 45.03
GKUE / HK / 1.79 / 3.31 / 2.18 A / 0.62 / 28.55 / 4.23 / 0.88 / 85.1
(g2GWP·mg-1GKC) / MK / 1.40 / 2.38 / 1.85 B / 0.46 / 24.97 / 3.35 / 0.94
LK / 1.42 / 2.30 / 1.99 AB / 0.56 / 28.08 / 3.47 / 0.76
StKUE / HK / 0.11 / 0.17 / 0.55 A / 0.12 / 21.17 / 1.01 / 0.32 / 80.1
(g2StWP·mg-1StKC) / MK / 0.14 / 0.13 / 0.59 A / 0.14 / 24.30 / 1.36 / 0.26
LK / 0.09 / 0.16 / 0.56 A / 0.13 / 23.35 / 0.92 / 0.29
Field tail
TGW / HK / 52.20 / 55.23 / 52.58 A / 5.70 / 10.84 / 63.43 / 34.40 / 96.6
(g) / MK / 48.20 / 56.89 / 51.54 A / 5.88 / 11.41 / 65.17 / 29.87
LK / 54.06 / 61.50 / 52.82 A / 6.23 / 11.43 / 68.41 / 34.92
GN / HK / 62.20 / 51.20 / 46.11 A / 6.48 / 14.05 / 67.40 / 32.80 / 86.5
(number) / MK / 64.20 / 49.60 / 46.27 A / 6.64 / 14.35 / 67.40 / 32.80
LK / 51.20 / 43.60 / 43.84 B / 7.04 / 16.05 / 63.20 / 27.40
SN / HK / 7.63 / 8.33 / 7.18 B / 1.77 / 24.68 / 11.44 / 3.00 / 73.9
(number) / MK / 6.86 / 11.00 / 7.69 A / 2.43 / 31.57 / 13.50 / 3.50
LK / 4.83 / 4.29 / 7.65 AB / 1.88 / 24.58 / 11.40 / 3.00
GWP / HK / 15.46 / 15.65 / 13.53 A / 5.76 / 42.53 / 29.77 / 3.16 / 83.3
(g·plant-1) / MK / 18.71 / 15.43 / 13.83 A / 5.15 / 37.21 / 26.24 / 2.49
LK / 15.34 / 15.02 / 13.93 A / 4.39 / 31.53 / 30.22 / 5.76
StWP / HK / 6.77 / 5.01 / 10.63 A / 4.70 / 44.23 / 26.60 / 2.33 / 82.8
(g·plant-1) / MK / 5.24 / 12.47 / 10.59 AB / 4.88 / 46.04 / 27.33 / 3.20
LK / 5.19 / 5.67 / 9.54 B / 3.04 / 31.87 / 20.83 / 2.10
GKC / HK / 72.30 / 75.97 / 64.22 A / 28.29 / 44.02 / 156.18 / 14.61 / 84.1
(mg·plant-1) / MK / 92.22 / 88.23 / 62.23 A / 23.16 / 37.22 / 127.70 / 11.99
LK / 72.57 / 81.05 / 55.70 B / 17.64 / 31.37 / 121.13 / 23.25
StKC / HK / 219.38 / 251.88 / 237.20 A / 119.70 / 50.64 / 699.02 / 49.71 / 76.3
(mg·plant-1) / MK / 115.37 / 278.91 / 228.77 A / 109.03 / 47.66 / 701.84 / 68.85
LK / 86.77 / 87.03 / 171.83 B / 56.37 / 33.04 / 306.77 / 37.34
GKUE / HK / 3.84 / 3.71 / 2.97 BC / 1.28 / 43.21 / 7.28 / 0.68 / 81.9
(g2GWP·mg-1GKC) / MK / 3.80 / 3.69 / 3.09 B / 1.26 / 40.68 / 6.65 / 0.44
LK / 3.95 / 3.76 / 3.47 A / 1.18 / 34.05 / 7.54 / 1.26
StKUE / HK / 0.21 / 0.17 / 0.48 B / 0.22 / 45.16 / 1.28 / 0.11 / 83.1
(g2StWP·mg-1StKC) / MK / 0.24 / 0.56 / 0.49 B / 0.23 / 47.50 / 1.15 / 0.14
LK / 0.31 / 0.37 / 0.55 A / 0.23 / 41.86 / 1.64 / 0.12

Table S3Analysis of variance (F values) of the RIL population for the investigated traits under hydroponic culture trial, pot trial and field trial

Hydroponic culture trial / Pot trial / Field trial
Traits / Source of variation / Traits / Source of variation / Traits / Source of variation
Genotypes / Treatments / Genotypes / Treatments / Genotypes / Treatments
RDW / 6.87** / 18.65** / TGW / 7.73** / 66.34** / TGW / 4.94** / 64.28**
SDW / 6.56** / 144.96** / GN / 3.83** / 39.71** / GN / 2.14** / 27.21**
RSDW / 7.24** / 314.21** / SN / 3.38** / 34.43** / SN / 1.38* / 17.50**
TDW / 6.63** / 114.54** / GWP / 3.00** / 21.91** / GWP / 1.81** / 4.73*
RKC / 4.01** / 414.31** / StWP / 2.70** / 65.32** / StWP / 1.80** / 19.45**
SKC / 4.33** / 397.44** / GKC / 2.01** / 30.49** / GKC / 1.90** / 23.05**
RSKC / 3.34** / 250.36** / StKC / 2.07** / 83.90** / StKC / 1.57** / 36.65**
TKC / 4.32** / 421.29** / GKUE / 2.56** / 50.70** / GKUE / 1.77** / 26.17**
RKUE / 2.41** / 311.52** / StKUE / 2.23** / 24.75** / StKUE / 1.82** / 18.22**
SKUE / 2.70** / 653.39**
TKUE / 2.69** / 789.94**

*, and ** indicate the significance at p≤0.05 and p≤0.01, respectively.

TableS4Spearmancorrelation coefficients (r) between investigated traits

TableS4-1 Spearmancorrelation coefficients (r) between investigated traitsunder hydroponic culture trial

Trait / RDW / SDW / RSDW / TDW / RKC / SKC / RSKC / TKC / RKUE / SKUE
SDW / .731**
RSDW / .219** / -.459**
TDW / .826** / .987** / -.326**
RKC / .346** / .688** / -.561** / .640**
SKC / .275** / .728** / -.706** / .657** / .941**
RSKC / .224** / .434** / -.335** / .405** / .835** / .648**
TKC / .286** / .723** / -.686** / .656** / .960** / .998** / .689**
RKUE / .224** / -.271** / .710** / -.171** / -.811** / -.799** / -.715** / -.809**
SKUE / .391** / -.032 / .582** / .064 / -.652** / -.686** / -.532** / -.689** / .908**
TKUE / .339** / -.138** / .664** / -.035 / -.724** / -.753** / -.608** / -.757** / .954** / .986**

*, and ** indicate the significance at p≤0.05 and p≤0.01, respectively.Df=655

Table S4-2 Spearmancorrelation coefficients (r) between investigated traits under pot trial

Trait / TGW / GN / SN / GWP / StWP / GKC / StKC / GKUE
GN / -.208**
SN / -.195** / -.094
GWP / .362** / .302** / .636**
StWP / .221** / .051 / .681** / .769**
GKC / .097 / .344** / .597** / .769** / .613**
StKC / .218** / .043 / .579** / .678** / .877** / .557**
GKUE / .458** / .178** / .432** / .809** / .602** / .299** / .521**
StKUE / .096 / .054 / .587** / .608** / .792** / .488** / .437** / .480**

*, and ** indicate the significance at p≤0.05 and p≤0.01, respectively. Df=392

Table S4-3 Spearmancorrelation coefficients (r) between investigated traits under field trial

Trait / TGW / GN / SN / GWP / StWP / GKC / StKC / GKUE
GN / -.199**
SN / -.033 / -.060
GWP / .152** / .140** / .576**
StWP / .037 / .076 / .403** / .640**
GKC / -.059 / .195** / .556** / .940** / .585**
StKC / .052 / .110* / .311** / .564** / .875** / .557**
GKUE / .168** / .072 / .564** / .951** / .615** / .801** / .513**
StKUE / .011 / .025 / .380** / .554** / .885** / .493** / .561** / .548**

*, and ** indicate the significance at p≤0.05 and p≤0.01, respectively.Df=392

Table S4-4 Spearmancorrelation coefficients (r) between seedling traits under hydroponic culture and traits of adult plant under pot trial and field trial

Trait / Trial / RDW / SDW / RSDW / TDW / RKC / SKC / RSKC / TKC / RKUE / SKUE / TKUE
TGW / Pot / .317** / .494** / -.260** / .462** / .256** / .514** / -.155 / .479** / -.144 / -.006 / -.124
Field / .214* / .293** / -.159 / .287** / .140 / .274** / -.046 / .261** / -.178* / .056 / -.047
GN / Pot / -.048 / -.151 / .163 / -.126 / -.027 / -.119 / .123 / -.104 / .040 / -.135 / -.099
Field / -.100 / -.227* / .194* / -.203* / -.146 / -.177* / -.005 / -.180* / .163 / -.203* / -.070
SN / Pot / -.150 / -.114 / -.149 / -.115 / -.166 / -.176* / -.137 / -.173 / .074 / .122 / .122
Field / -.024 / .015 / -.077 / .010 / -.042 / -.079 / .023 / -.070 / .070 / .251** / .224*
GWP / Pot / .115 / .226* / -.267** / .212* / .080 / .221* / -.148 / .208* / -.059 / .026 / -.074
Field / .058 / .178* / -.173 / .177* / -.033 / .097 / -.118 / .077 / .114 / .183* / .195*
StWP / Pot / .075 / .215* / -.279** / .194* / .025 / .171 / -.171 / .153 / -.089 / .178* / .088
Field / .130 / .138 / -.019 / .142 / .078 / .080 / .014 / .081 / .019 / .147 / .152
GKC / Pot / .001 / .047 / -.156 / .045 / .003 / .061 / -.122 / .064 / .108 / -.073 / -.121
Field / -.010 / .063 / -.143 / .049 / -.091 / .023 / -.120 / .003 / .165 / .146 / .179*
StKC / Pot / .089 / .215* / -.269** / .198* / .058 / .174 / -.132 / .167 / -.064 / .157 / .065
Field / .091 / .110 / -.027 / .110 / .009 / .065 / -.028 / .052 / .049 / .112 / .141
GKUE / Pot / .217* / .340** / -.257** / .326** / .161 / .318** / -.118 / .298** / -.175* / .091 / -.014
Field / .113 / .219* / -.166 / .204* / .007 / .155 / -.137 / .131 / .067 / .221* / .221*
StKUE / Pot / .032 / .155 / -.198* / .134 / -.016 / .110 / -.145 / .089 / -.084 / .140 / .091
Field / .104 / .112 / -.024 / .114 / .107 / .088 / .028 / .096 / .001 / .093 / .086

*, and ** indicate the significance at p≤0.05 and p≤0.01, respectively.P and F mean pot trial and field trial respectively. Df=130.

Table S5Additive QTLs detected in different K treatments under hydroponic culture, pot trial and field trial.

Trait / QTL / Treatment / Marker interval a / Sitesb
(cM) / LODs / Additive effectsc / Contributions
(%)
Hydroponic culture
RDW / QHrdw-1A.1 / MK / wPt-6358-wPt-730618 / 0 / 4.96 / 1.57 / 13.58
LK1 / wPt-669484-wPt-6358 / 1 / 3.87 / 1.56 / 10.72
LK2 / wPt-669484-wPt-6358 / 1 / 5.74 / 1.89 / 15.90
QHrdw-1A.2 / HK / wPt-8770-wPt-731476 / 0 / 3.97 / -2.63 / 10.57
QHrdw-2B / LK1 / wPt-6223-wPt-8460 / 3 / 3.29 / -1.56 / 10.18
LK2 / wPt-6223-wPt-8460 / 13 / 5.19 / -2.75 / 33.56
QHrdw-6A / LK2 / wPt-671568-wPt-666964 / 0 / 3.51 / 1.42 / 8.75
QHrdw-6D / LK1 / wPt-667726-wPt-668152 / 0 / 5.56 / -1.84 / 14.26
SDW / QHsdw-1A.1 / HK / wPt-669484-wPt-6358 / 1 / 6.82 / 6.74 / 20.38
LK2 / wPt-669484-wPt-6358 / 1 / 3.11 / 4.10 / 9.06
QHsdw-1A.2 / MK / wPt-730172-wPt-669607 / 9 / 5.42 / -8.12 / 17.88
QHsdw-4A / HK / sarp18-issr23b / 0 / 3.75 / 8.81 / 9.91
LK2 / swes624c-sarp18 / 5 / 3.32 / 7.86 / 9.12
QHsdw-4B / LK3 / swes24a-swes24c / 1 / 3.56 / -3.41 / 10.19
QHsdw-7D / LK1 / wPt-8422-wPt-731386 / 0 / 3.13 / 4.38 / 7.89
RSDW / QHrsdw-1A / LK2 / wPt-664666-wmc312a / 3 / 6.12 / -0.01 / 13.86
LK3 / wPt-664666-wmc312a / 10 / 5.62 / -0.02 / 15.83
QHrsdw-1B.1 / LK1 / wmc128-swes189 / 0 / 3.31 / 0.01 / 7.88
QHrsdw-1B.2 / MK / ubc834b-ubc880d / 4 / 3.10 / -0.01 / 7.88
QHrsdw-2A / MK / barc220-swes598 / 3 / 4.70 / -0.01 / 10.81
LK2 / barc220-swes598 / 1 / 4.90 / -0.01 / 10.08
QHrsdw-3B.1 / HK / issr25a-sarp5c / 0 / 5.15 / 0.01 / 11.35
MK / issr25a-sarp5c / 0 / 3.08 / 0.01 / 6.61
QHrsdw-3B.2 / LK3 / gwm547-gwm340 / 12 / 4.75 / 0.01 / 11.46
QHrsdw-5B / HK / issr22b-ubc847a / 0 / 4.47 / -0.01 / 9.90
QHrsdw-5D / LK1 / sarp6b-sarp20 / 5 / 5.63 / -0.01 / 21.83
TDW / QHtdw-1A.1 / HK / wPt-669484-wPt-6358 / 2 / 3.77 / 6.07 / 10.46
LK1 / wPt-669484-wPt-6358 / 1 / 4.11 / 6.32 / 11.21
LK2 / wPt-669484-wPt-6358 / 1 / 3.98 / 6.2 / 11.62
QHtdw-1A.2 / MK / wPt-669607-wPt-665590 / 0 / 4.89 / -9.28 / 14.55
QHtdw-2B / LK2 / wPt-6223-wPt-8460 / 16 / 4.48 / -10.9 / 36.84
QHtdw-3A / HK / wPt-730892-barc314 / 3 / 3.04 / 31.96 / 13.26
QHtdw-4A / HK / sarp18-issr23b / 0 / 4.10 / 12.08 / 11.06
QHtdw-6A / LK2 / wPt-672030-wPt-671568 / 6 / 3.27 / 5.50 / 8.98
QHtdw-6D / LK1 / wPt-667726-wPt-668152 / 0 / 4.71 / -6.62 / 11.91
RKC / QHrkc-1A / MK / wPt-669484-wPt-6358 / 2 / 4.58 / 0.07 / 13.03
LK1 / wPt-669484-wPt-6358 / 1 / 3.14 / 0.04 / 7.96
LK2 / wPt-669484-wPt-6358 / 0 / 5.03 / 0.02 / 11.69
QHrkc-3B / LK3 / wPt-0751-wPt-7614 / 0 / 3.24 / 0.01 / 8.14
QHrkc-4B / LK3 / swes24a-swes24c / 0 / 4.44 / -0.02 / 11.22
QHrkc-5D / LK2 / sarp6b-sarp20 / 17 / 3.76 / -0.03 / 26.44
QHrkc-6A / LK2 / wPt-671568-wPt-666964 / 0 / 4.98 / 0.02 / 11.66
QHrkc-6D / LK1 / wPt-667726-wPt-668152 / 0 / 3.89 / -0.04 / 9.41
SKC / QHskc-1A.1 / MK / wPt-669484-wPt-6358 / 0 / 3.37 / 0.22 / 7.95
LK2 / wPt-669484-wPt-6358 / 1 / 4.05 / 0.11 / 10.92
LK3 / wPt-6358-wPt-730618 / 0 / 3.29 / 0.06 / 9.25
QHskc-1D / LK1 / wmc432b-wPt-665480 / 9 / 3.20 / -0.16 / 9.78
QHskc-2B / MK / wmc154a-wmc154b / 5 / 5.01 / 0.30 / 12.91
QHskc-3A / MK / wPt-730892-barc314 / 0 / 4.58 / 0.6 / 11.38
QHskc-4A / HK / sarp18-issr23b / 0 / 3.88 / 0.45 / 10.23
LK2 / sarp18-issr23b / 0 / 5.16 / 0.24 / 12.63
QHskc-4B / LK3 / wPt-5334-swes1117 / 0 / 3.77 / -0.06 / 10.70
QHskc-7A / HK / barc121-ubc873c / 5 / 3.20 / -0.22 / 8.41

aMarker interval isthe interval of LOD peak value for QTLs.

bSite isthe distance of LOD peak value for QTL after the first marker in the marker interval.

c Positive additive effect is the increased effect contributed by Chuang 35050; negative effect iscontributed by Shannong 483

Table S5 Continued

Trait / QTL / Treatment / Marker intervala / Sites b
(cM) / LODs / Additive effectsc / Contributions (%)
Hydroponic culture
RSKC / QHrskc-1A / MK / wmc312b-GluA1 / 5 / 4.51 / -0.02 / 16.54
QHrskc-3A.1 / LK3 / ubc859e-wPt-730892 / 4 / 5.71 / 0.03 / 22.12
QHrskc-3A.2 / LK2 / wPt-668205-swes1157 / 1 / 3.59 / 0.01 / 10.36
QHrskc-3B.1 / LK3 / ubc860b-wPt-5836 / 4 / 3.41 / -0.01 / 10.23
QHrskc-3B.2 / HK / wPt-2416-issr25a / 9 / 3.03 / 0.01 / 9.64
QHrskc-4A.1 / LK1 / swes147-swes624c / 1 / 3.27 / 0.02 / 14.26
QHrskc-4A.2 / MK / wmc308-wmc513 / 1 / 3.86 / 0.06 / 13.32
QHrskc-4B.1 / LK3 / swes24a-swes24c / 1 / 4.69 / -0.01 / 12.24
QHrskc-4B.2 / LK1 / wPt-5334-swes1117 / 6 / 3.21 / 0.01 / 10.52
QHrskc-5D / LK2 / sarp6b-sarp20 / 9 / 4.91 / -0.01 / 25.57
QHrskc-7A / MK / wPt-667328- wPt-668064 / 0 / 3.83 / -0.01 / 9.68
TKC / QHtkc-1A / MK / wPt-669484-wPt-6358 / 0 / 4.20 / 0.30 / 10.38
LK1 / wPt-669484-wPt-6358 / 1 / 3.75 / 0.19 / 9.90
LK2 / wPt-669484-wPt-6358 / 0 / 4.93 / 0.13 / 11.39
QHtkc-2B.1 / MK / wmc154b-wPt-7970 / 0 / 4.93 / 0.35 / 12.82
QHtkc-2B.2 / LK2 / wPt-6223-wPt-8460 / 12 / 4.16 / -0.18 / 21.89
QHtkc-3A / MK / wPt-730892-barc314 / 0 / 3.73 / 0.65 / 9.46
QHtkc-3B / LK3 / wPt-0751-wPt-7614 / 2 / 3.09 / 0.07 / 9.64
QHtkc-4A / HK / sarp18-issr23b / 0 / 4.31 / 0.59 / 11.20
LK2 / sarp18-issr23b / 0 / 4.05 / 0.22 / 8.94
QHtkc-4B / LK3 / swes24b-swes24a / 7 / 6.08 / -0.10 / 18.79
QHtkc-5B / LK1 / wPt-9814-wPt-7114 / 18 / 3.36 / 0.18 / 9.16
QHtkc-5D / LK3 / sarp6b-sarp20 / 28 / 3.58 / -0.09 / 13.3
QHtkc-6A / LK2 / wPt-672030-wPt-671568 / 5 / 4.68 / 0.14 / 12.37
QHtkc-6B / LK1 / wPt-5176-wPt-1541 / 0 / 3.68 / 0.18 / 8.83
QHtkc-6D / LK1 / wPt-667726-wPt-668152 / 0 / 3.85 / -0.18 / 9.13
QHtkc-7A / HK / ubc859a-swes624e / 10 / 3.80 / -0.36 / 14.83
RKUE / QHrkue-1A.1 / HK / wPt-730902-wPt-731490 / 5 / 3.71 / 0.04 / 12.50
QHrkue-1A.2 / HK / wPt-8770-wPt-731476 / 1 / 4.42 / -0.07 / 12.66
QHrkue-1B / HK / ubc834b-ubc880d / 3 / 3.22 / -0.04 / 9.05
QHrkue-1D / LK2 / wmc336b-wmc432b / 9 / 3.49 / -0.28 / 11.89
QHrkue-2B / LK1 / wPt-0100-wPt-6627 / 7 / 3.04 / -0.07 / 8.89
QHrkue-3A.1 / HK / wPt-730892-barc314 / 2 / 3.55 / 0.17 / 21.25
MK / wPt-730892-barc314 / 2 / 4.52 / 0.18 / 23.75
QHrkue-3A.2 / MK / wPt-668205-swes1157 / 2 / 4.39 / 0.08 / 20.42
QHrkue-4A / LK1 / srawPt-7b-srawPt-7c / 3 / 3.78 / 0.18 / 11.88
QHrkue-5B / HK / wPt-665267-wPt-6348 / 0 / 5.25 / 0.06 / 13.50
QHrkue-7A / MK / gwm471-wmc497a / 4 / 3.05 / -0.15 / 22.60
QHrkue-7B.1 / MK / wPt-3533-swes19 / 15 / 4.32 / 0.05 / 14.90
QHrkue-7B.2 / LK3 / wPt-2356-wPt-3093 / 1 / 4.85 / 0.66 / 14.01
QHrkue-7D / MK / wPt-8422-wPt-731386 / 49 / 3.29 / 0.04 / 9.03
LK1 / wPt-731386-wPt-665461 / 0 / 3.31 / 0.07 / 8.53
SKUE / QHskue-3B.1 / LK2 / wPt-6223-wPt-8460 / 27 / 3.03 / -0.36 / 35.30
QHskue-3B.2 / MK / wPt-1940-wmc418 / 0 / 3.75 / -0.13 / 10.38
QHskue-7B / HK / wPt-2305-wPt-7887 / 1 / 3.72 / -0.16 / 11.54
QHskue-7D / LK2 / wPt-8343-wPt-4555 / 0 / 3.33 / 0.21 / 9.60
TKUE / QHtkue-1A.1 / LK1 / wPt-669484-wPt-6358 / 1 / 4.33 / 0.23 / 13.15
QHtkue-1A.2 / LK2 / wPt-729788-wPt-667395 / 0 / 4.31 / -0.50 / 12.58
QHtkue-1A.3 / HK / wPt-8770-wPt-731476 / 1 / 3.39 / -0.25 / 11.32
LK2 / wPt-730213-wPt-8770 / 1 / 3.83 / -0.55 / 11.23
LK3 / wPt-730213-wPt-8770 / 1 / 4.03 / -0.62 / 12.18
QHtkue-3A / MK / wPt-668205-swes1157 / 0 / 6.24 / 0.34 / 17.52
QHtkue-4B / LK3 / swes24a-swes24c / 0 / 4.18 / -0.39 / 11.62
QHtkue-5B / LK3 / barc59a-swes1067 / 0 / 3.18 / -0.32 / 8.65
QHtkue-7B / MK / swes19-wPt-6156 / 0 / 4.13 / 0.17 / 11.59
QHtkue-7D / MK / wPt-8422-wPt-731386 / 0 / 3.05 / 0.15 / 7.88
LK1 / wPt-731386-wPt-665461 / 0 / 3.79 / 0.23 / 11.29
LK2 / wPt-8343-wPt-4555 / 0 / 3.68 / 0.33 / 10.28

Table S5 Continued

Trait / QTL / Treatment / Marker intervala / Sitesb
(cM) / LODs / Additive effectsc / Contributions (%)
Pot trial
TGW / QPtgw-1A.1 / HK / wPt-730902-wPt-731490 / 20 / 3.37 / 1.72 / 7.13
QPtgw-1A.2 / LK / wmc312a-wmc93a / 1 / 3.98 / 2.00 / 9.14
QPtgw-1B / MK / swes1109b-gdm28 / 24 / 3.19 / -2.17 / 12.03
QPtgw-1D.1 / HK / wmc222-wmc432a / 13 / 3.70 / 2.30 / 12.03
QPtgw-1D.2 / MK / wmc432b-wPt-665480 / 9 / 5.88 / -2.43 / 14.61
LK / wmc432b-wPt-665480 / 7 / 8.51 / -3.29 / 25.58
QPtgw-2B.1 / MK / wmc154a-wmc154b / 1 / 3.76 / 1.93 / 7.84
QPtgw-2B.2 / MK / wPt-0047-wmc149a / 12 / 3.60 / 1.89 / 8.68
QPtgw-3A / HK / wPt-666438-wPt-4692 / 4 / 5.04 / 2.40 / 14.23
MK / wPt-666438-wPt-4692 / 4 / 9.60 / 3.04 / 24.09
QPtgw-4A.2 / MK / wPt-9183-wPt-8167 / 0 / 3.03 / -1.58 / 5.72
QPtgw-4A.1 / LK / wPt-0032-wPt-4620 / 1 / 3.02 / -1.77 / 6.55
QPtgw-4B / HK / swes24c-wPt-7569 / 17 / 3.85 / -2.14 / 10.12
GN / QPgn-1A / HK / wPt-669607-wPt-665590 / 18 / 4.79 / -3.17 / 20.10
QPgn-1B / HK / wPt-668027-swes169a / 7 / 3.12 / -3.2 / 17.29
QPgn-2A / MK / barc220-swes598 / 3 / 8.56 / -2.93 / 20.41
LK / gwm95-barc208 / 0 / 3.53 / -2.35 / 9.27
QPgn-2D / HK / issr23a-wmc181b / 0 / 5.59 / 2.49 / 13.10
MK / wmc170b-ubc826a / 3 / 9.66 / 3.74 / 23.41
QPgn-3A.1 / HK / wPt-731120-wPt-666438 / 9 / 6.91 / -3.00 / 21.03
QPgn-3A.2 / MK / wPt-668205-swes1157 / 0 / 5.04 / -2.84 / 10.58
QPgn-4B / MK / wPt-7569-wPt-3991 / 0 / 3.48 / 1.63 / 7.20
QPgn-5D / LK / swes340a-swes342a / 6 / 3.44 / -2.51 / 10.66
SN / QPsn-1B / MK / wPt-6427-wPt-668027 / 5 / 4.22 / 0.62 / 11.81
QPsn-2B / LK / wPt-9350-barc18b / 10 / 3.03 / -0.35 / 11.42
QPsn-5A / LK / sarp9-wPt-1370 / 6 / 3.02 / 0.36 / 11.46
QPsn-6A / MK / wmc256-wPt-665784 / 1 / 3.16 / -0.50 / 9.54
GWP / QPgwp-1D / MK / wPt-666067-wPt-4647 / 2 / 3.35 / -0.47 / 8.93
QPgwp-2D / HK / wPt-730613-wPt-9749 / 0 / 4.48 / 0.51 / 10.64
QPgwp-6A / HK / wPt-731524-wPt-7204 / 7 / 3.26 / 0.43 / 8.66
StWP / QPstwp-1A / HK / wPt-730213-wPt-8770 / 0 / 3.70 / -0.88 / 9.25
QPstwp-1B / LK / ubc849-swes1106b / 10 / 5.08 / 0.85 / 17.92
QPstwp-2D / HK / wPt-730613-wPt-9749 / 0 / 5.45 / 0.71 / 13.70
QPstwp-4A.1 / MK / wPt-0610-wPt-730913 / 7 / 5.39 / 1.11 / 14.99
QPstwp-4A.2 / MK / wPt-671707-barc70a / 0 / 7.61 / -1.18 / 19.83
QPstwp-5A / LK / swes332b-swes1035b / 1 / 3.05 / -0.59 / 8.41
QPstwp-7D / MK / wmc121a-wmc121b / 6 / 4.28 / 0.94 / 14.48
GKC / QPgkc-2A / LK / wPt-668044-wPt-731406 / 0 / 4.09 / 2.42 / 10.64
QPgkc-2B.1 / MK / wmc154b-wPt-7970 / 28 / 4.20 / 2.74 / 11.55
QPgkc-2B.2 / HK / wPt-0643-wPt-6158 / 0 / 3.69 / -3.02 / 10.57
QPgkc-3A / MK / wPt-668205-swes1157 / 0 / 5.2 / -4.10 / 12.81
StKC / QPstkc-2D / HK / wPt-2544-wPt-730613 / 6 / 4.66 / 23.83 / 13.43
GKUE / QPgkue-1A / LK / wPt-1973-wPt-671790 / 0 / 3.15 / 0.29 / 9.79
QPgkue-1D / MK / wPt-665480-wPt-666067 / 5 / 7.08 / -0.22 / 20.27
QPgkue-2D / HK / ubc815b-wmc445b / 8 / 5.99 / 0.30 / 16.98
QPgkue-7D / MK / wPt-8343-wPt-4555 / 0 / 3.25 / 0.14 / 7.66
StKUE / QPstkue-2B / HK / wPt-8460-wPt-9350 / 0 / 3.55 / -0.04 / 8.76
QPstkue-2D / LK / wmc170b-ubc826a / 1 / 3.26 / 0.04 / 8.77
QPstkue-4A.1 / MK / wPt-0610-wPt-730913 / 6 / 5.88 / 0.09 / 19.77
QPstkue-4A.2 / MK / wPt-671707-barc70a / 1 / 7.17 / -0.09 / 21.64
QPstkue-5A / LK / wPt-3620-swes25 / 5 / 5.26 / 0.06 / 18.81
QPstkue-5B / HK / wPt-1302-wPt-6498 / 4 / 4.83 / -0.06 / 12.66
QPstkue-5D / LK / gwm182-gdm43 / 0 / 3.07 / 0.05 / 8.03
QPstkue-7A / HK / wPt-667328-wPt-668064 / 0 / 4.52 / 0.04 / 11.59
QPstkue-7D / MK / wmc121a-wmc121b / 0 / 3.63 / 0.05 / 11.48

Table S5 Continued

Trait / QTL / Treatment / Marker intervala / Sitesb
(cM) / LODs / Additive effectsc / Contributions (%)
Field trial
TGW / QFtgw-2B / MK / wPt-0047-wmc149a / 2 / 4.89 / 2.19 / 12.28
QFtgw-3A / LK / wPt-666438-wPt-4692 / 0 / 3.16 / 1.80 / 7.86
MK / wPt-666438-wPt-4692 / 0 / 3.99 / 1.80 / 8.90
QFtgw-6A / HK / wPt-672030-wPt-671568 / 1 / 6.81 / 2.42 / 16.45
MK / wPt-668031-wPt-4229 / 6 / 7.52 / 2.78 / 19.31
LK / wPt-5696-wPt-672030 / 4 / 4.94 / 2.36 / 12.94
QFtgw-6B / HK / wPt-8015-wPt-5234 / 0 / 6.38 / -2.27 / 14.62
LK / swes1106a-wPt-8015 / 8 / 4.11 / -2.26 / 12.21
QFtgw-7A / HK / wPt-6668-gwm635 / 2 / 6.28 / 2.35 / 15.80
QFtgw-7B / HK / wPt-1066-wPt-0194 / 6 / 3.32 / 2.63 / 9.73
GN / QFgn-1A / HK / swes131b-swes131a / 0 / 5.28 / -2.92 / 14.75
QFgn-2A / MK / ubc811c-ubc873b / 0 / 3.16 / -1.94 / 7.97
QFgn-3B / LK / sarp7a-swes862 / 0 / 3.46 / -2.14 / 8.26
QFgn-4A / HK / sarp18-issr23b / 2 / 3.22 / 6.54 / 19.6
LK / sarp18-issr23b / 1 / 5.92 / 6.18 / 19.09
QFgn-7B / MK / wPt-3093-wPt-669526 / 4 / 7.24 / 4.57 / 26.65
SN / QFsn-1B / MK / swes1079b-swes1079a / 2 / 3.38 / 0.79 / 9.55
QFsn-3D / HK / wmc529-sarp8 / 0 / 3.11 / 0.87 / 10.22
QFsn-6A / MK / wPt-3247-wPt-1695 / 0 / 3.10 / -0.73 / 7.74
QFsn-7D / LK / wmc121a-wmc121b / 11 / 4.37 / -1.09 / 31.57
GWP / QFgwp-2D / HK / wPt-2544-wPt-730613 / 1 / 3.03 / 1.89 / 9.64
QFgwp-6B / MK / wPt-4924-wPt-3060 / 8 / 6.67 / -2.74 / 27.00
StWP / QFstwp-1D / HK / wPt-4647-swes1100 / 15 / 4.23 / -2.30 / 22.83
QFstwp-3D / MK / gwm645-wmc56 / 0 / 4.36 / -2.20 / 14.55
GKC / QFgkc-2B / MK / wPt-3561-wPt-5374 / 5 / 3.10 / -0.79 / 10.24
QFgkc-5B / HK / wmc3b-swes430a / 7 / 4.18 / -1.48 / 15.98
QFgkc-6B / MK / wPt-4924-wPt-3060 / 12 / 5.05 / -1.10 / 20.57
QFgkc-7D / LK / wPt-8422-wPt-731386 / 0 / 3.55 / -0.58 / 9.52
StKC / QFstkc-1B / LK / wmc419b-cfd20 / 0 / 3.49 / 2.09 / 10.29
QFstkc-2A / MK / barc15-wmc445c / 0 / 3.31 / -4.88 / 10.98
QFstkc-2B / LK / wPt-9736-wPt-9736 / 0 / 6.57 / -4.05 / 20.24
GKUE / QFgkue-1A / LK / wmc93a-wmc312b / 3 / 5.77 / 0.57 / 18.66
QFgkue-6B / MK / wPt-4924-wPt-3060 / 3 / 4.16 / -0.52 / 15.40
StKUE / QFstkue-6A / HK / wPt-666494-wPt-3247 / 3 / 3.95 / -0.09 / 12.50