Supplementary Table S1. Primers used in this study for construction of a gene disruption vector. Underlined sequences show restriction enzyme sites. The 15 bp homologous ends used for in-fusion cloning are shown in italics.

No. / Primer / Sequence (5’à3’) / Purpose
1 / FoAvt3-F / CGGGATCCATGGCTGAGTTCGCCAATTTCG / FoAVT3 ORF
2 / FoAvt3-R / GGAATTCCTAAGCACTGTCGATCCACTG
3 / TrpCP-F / ATGCCAGTTGTTCCAGTGATCTTCGTTTCG / A. nidulans
TrpC promoter
4 / TrpCP-R / CTCGAGTGGAGATGTGGAGTGGGCGCTTAC
5 / TrpCT-F / CCGCGGCCATGGTGATTTAATAGCTCCATGTCAACAAGAATA / A. nidulans TrpC terminator
6 / TrpCT-R / GACGTCGCATGCTGGAGATGTGGAGTGGGCGCTTACACAGTA
7 / Vec-TrpCT-F / TAGTGATTTAATAGCTCCATGTCAACAAGA / linearizing vector
8 / Vec-TrpCP-R / GATCGAGGCTTGGGTAGAATAGGTAAGTCA
9 / HPH-F / ACCCAAGCCTCGATCATGAAAAAGCCTGAACTCACCGCGACGTCT / hph*
10 / HPH-R / GCTATTAAATCACTATTCCTTTGCCCTCGGACGAGTGCTGGGGCG
11 / Neo-F / ACCCAAGCCTCGATCATGATTGAACAAGATGGATTGCACGCAGGT / npt II**
12 / Neo-R / GCTATTAAATCACTAGAAGAACTCGTCAAGAAGGCGATAGAAGGC
13 / Marker-F / CCCAAGCGGCCGCATGCCAGTTGTTCCAGTGATCTTCGTTTCG / selection marker
142 / Marker-R / CCCAAGCGGCCGCTGGAGATGTGGAGTGGGCGCTTACACAGTA
15 / FoAvt3P-F / CTGCGTGCCTGAGGAGAAAATTTGCCATAC / FoAVT3†
16 / FoAvt3T-R / TACTTGGAGGAGCATATGATGGAAAGTGAG
17 / EGFP-F / TGACAATCCACAATCATGGTGAGCAAGGGCGAGGAGCTGTTCACC / EGFP††
18 / EGFP-R / GGCGAACTCAGCCATCTTGTACAGCTCGTCCATGCCGAGAGTGAT
19 / GpdAP-F / AAAAGTCACACAACACAAGCTGTAAGGATT / A. nidulans
GpdA promoter
20 / GpdAP-R / TGTGATGTCTGCTCAAGCGGGGTAGCTGTT
21 / GpdAT-F / CCGCGGCCATGGTAGGAAACAGGTCGGAAGCCAATGG / A. nidulans
GpdA terminator
22 / GpdAT-R / GACGTCGCATGCAGTCAAGTAAGAATGACTGAGGTGCGC
23 / Vec-GpdAT-F / GAAACAGGTCGGAAGCCAATGGCCAGGAGC / linearizing vector
24 / Vec-GpdAP-R / TGTGATGTCTGCTCAAGCGGGGTAGCTGTT
25 / GpdA-EGFP-F / TGAGCAGACATCACAATGGTGAGCAAGGGCGAGGAG / EGFP-FOAVT3
fusion
26 / GpdA-FoAvt3-R / CTTCCGACCTGTTTCCTAAGCACTGTCGATCCACTGGTAAACAGT

* hygromycin B phosphotransferase gene

** neomycin phosphotransferase gene

† 1 kbp upstream and downstream of FoAvt3 open reading frame

†† EGFP to insert between end of promoter and start codon of FoAvt3

Supplementary Table S2. Oligonucleotide primers used in this study for RT-PCR.

No. / Primer / Sequence (5’à3’) / Purpose
1 / α-tubulin-F / TTGGACAGGCTGGTTGCCAAATCGCCAACT / tubulin α chain FOXG_00956
2 / α-tubulin-F / GCGATGGCGGTAGTATTAGAGAGCATGCAG
3 / FoAvt3-F / ATGGCTGAGTTCGCCAATTTCGACGGAACA / FoAVT3
FOXG_11334
4 / FoAvt3-R / ACCTAGTTCTCCATAACCGCCTC
5 / FoMtd1-F / CCTATCTTCCAGATGTTGGCTGGC / peptide transporter
FOXG_16609
6 / FoMtd1-R / CCGACGAAAAGGTAACCGAGGTACC
7 / FoAap1-F / CGAGTTGATCGCTGCGTCAAAGAC / amino acid permease FOXG_08912
8 / FoAap1-R / CCTTTGTGTTGGCGCCAGAAGC
9 / FoAvt4-F / ATTCGGGAACACCGACCAACTATGGCTCTT / FoAVT4
FOXG_07895
10 / FoAvt4-R / GAGGAAGCTTGAGGTGAACAATCTCTGCTG