Electronic Supplementary Material
Fecal Bacterial Composition of Sichuan Snub-Nosed Monkeys (Rhinopithecus roxellana)
Chun Su ∙ Renjuan Zuo ∙ Wenwang Liu ∙ Yan Sun ∙ Zhi Li ∙ Xuelin Jin ∙ Kangsheng Jia ∙ Yi Yang ∙ Hongjuan Zhang
Table SI Results of BLAST search of the 12 bands excised from the DGGE gel shown in Fig. 1
Band no. / Closest relatives / Similarity (%)1 / Uncultured bacterium clone J135 (GQ451227.1) / 98
2 / Uncultured bacterium clone DF9269 (GU617445.1) / 100
Uncultured Lachnospiraceae bacterium clone (EF706798.1) / 99
3 / Uncultured bacterium clone (FJ505998.1) / 98
Bacteroides sp. Smarlab 3302398 (AY538696.1) / 98
4 / Uncultured bacterium clone (GU579469.1) / 100
5 / Uncultured bacterium clone (EU475560.1) / 95
6 / Uncultured bacterium clone TRT43 (FJ032524.1) / 99
Treponema berlinense strain 7CPL208 (AY230217.1) / 99
7 / Uncultured bacterium gene for (AB606394.1) / 100
8 / Clostridiales sp. SM4/1 draft genome (FP929060.1) / 100
Uncultured Firmicutes bacterium clone (GU959352.1) / 100
9 / Uncultured bacterium clone 5-8E12 (FJ681735.1) / 100
10 / Uncultured bacterium clone J39 (GQ451194.1) / 99
Uncultured Bacteroidetes bacterium clone (HM105152.1) / 95
11 / Uncultured bacterium clone RT_aai10h06 (EU778577.1) / 99
Uncultured bacterium clone RT_aai10h06 (EU778577.1) / 99
12 / Uncultured bacterium clone J271 (GQ451276.1) / 99
Table SII Results of BLAST search of the 34 bands excised from the DGGE gel shown in Fig. 3
1 / Uncultured Bacteroidetes bacterium clone CTF2-183 16S ribosomal RNA gene (GU958265.1) / 97
2 / Uncultured Bacteroidetes bacterium clone CM2-131 16S ribosomal RNA gene (GU959289.1) / 95
3 / Uncultured bacterium clone Hma1-23 16S ribosomal RNA gene (JX096049.1) / 97
4 / Uncultured organism clone ctg_CGOAA39 16S ribosomal RNA gene (DQ395479.1) / 96
5 / Uncultured bacterium clone calf32_6wks_grp5_D12 16S ribosomal RNA gene (GQ448887.1) / 100
6 / Uncultured bacterium clone FS2_16S_127 16S ribosomal RNA gene (JQ336643.1) / 93
7 / Uncultured bacterium clone T2C251 16S ribosomal RNA gene (JQ265541.1) / 100
8 / Uncultured bacterium clone Hma1-23 16S ribosomal RNA gene (JX509218.1) / 97
9 / Uncultured Lachnospiraceae bacterium clone L-123 16S ribosomal RNA gene (JX543482.1) / 98
10 / Uncultured Firmicutes bacterium clone p1o23pool 16S ribosomal RNA gene, (HM104982.1) / 100
11 / Solobacterium moorei strain C1107 16S ribosomal RNA gene (JF803577.1) / 95
12 / Uncultured Firmicutes bacterium gene for 16S rRNA, partial sequence, clone: Ola1.D12.invm13r (AB691190.1) / 99
13 / Uncultured rumen bacterium gene for 16S rRNA, partial sequence, clone: F23-H06 (AB185568.1) / 99
14 / Uncultured organism clone ELU0116-T290-S-NI_000218 small subunit ribosomal RNA gene (HQ789437.1) / 97
15 / Uncultured Bacteroidetes bacterium clone CM1-160 16S ribosomal RNA gene (GU958300.1) / 97
16 / Uncultured Veillonellaceae bacterium clone BLUC-A 16S ribosomal RNA gene (DQ196617.1) / 95
17 / Uncultured bacterium partial 16S rRNA gene, clone 16sps27-5e01.p1k (FM997721.1) / 96
18 / Uncultured Veillonellaceae bacterium clone BLUC-A 16S ribosomal RNA gene (DQ196617.1) / 95
19 / Uncultured bacterium clone 12m0215029 16S ribosomal RNA gene (JX548116.1) / 100
20 / Uncultured bacterium clone J305 16S ribosomal RNA gene (GQ451297.1) / 98
21 / Uncultured Clostridium sp. partial 16S rRNA gene, clone ROM_10 (HE575392.1) / 100
22 / Uncultured Veillonellaceae bacterium clone BLUC-A 16S ribosomal RNA gene (DQ196617.1) / 95
23 / Uncultured Firmicutes bacterium clone CTF1-41 16S ribosomal RNA gene (GU958943.1) / 97
24 / Uncultured bacterium clone BB1_c04_1 16S ribosomal RNA gene (EU467843.1) / 100
25 / Uncultured bacterium clone OK1_a04 16S ribosomal RNA gene (EU468457.1) / 100
26 / Uncultured bacterium clone L-188 16S ribosomal RNA gene (EU622688.2) / 95
27 / Uncultured bacterium clone CHIMP1_aaj37b02 16S ribosomal RNA gene (EU462199.1) / 100
28 / Uncultured Lachnospiraceae bacterium clone MS120A1_E08 16S ribosomal RNA gene (EF704311.1) / 99
29 / Uncultured bacterium clone AFBAB_aai04d05 16S ribosomal RNA gene (EU464400.1) / 100
30 / Uncultured bacterium clone ZQ-72 16S ribosomal RNA gene (JX173826.1) / 95
31 / Uncultured bacterium clone calf784_6wks_grp2_D01 16S ribosomal RNA gene (GQ449167.1) / 100
32 / Uncultured bacterium clone GOR_aag72a09 16S ribosomal RNA gene (EU469833.1) / 99
33 / Uncultured bacterium clone CHIMP12_f06 16S ribosomal RNA gene (EU774372.1) / 98
34 / Uncultured bacterium clone 12m0215029 16S ribosomal RNA gene (JX548116.1) / 100
Table SIII Results of BLAST search of the 71 OTUs from the 16S rRNA library
1 / Uncultured bacterium clone RL186 aan90b06 (DQ806301.1) / 95
2 / Uncultured bacterium clone CNSL-03 (AB507592.1) / 89
3 / Uncultured bacterium clone 935 (AJ863522.1) / 90
4 / Uncultured bacterium clone J39 (GQ451194.1) / 91
5 / Uncultured bacterium clone J39 (GQ451194.1) / 99
6 / Phascolarctobacterium sp. canine oral taxon 149 clone PP041 (JN713316.1) / 92
7 / Uncultured bacterium clone CA_254 (JN559684.1) / 94
8 / Uncultured bacterium clone FL_1aaa02a04 (EU775101.1) / 90
9 / Uncultured bacterium (EU778040.1) / 92
10 / Uncultured bacterium (AB627501.1) / 94
11 / Uncultured Ruminococcaceae bacterium (EU794170.1) / 95
12 / Uncultured bacterium (JX096132.1) / 97
13 / Uncultured bacterium (JX095622.1) / 89
14 / Parabacteroides distasonis strain JCM5825 (EU136681.1) / 94
15 / Uncultured bacterium clone R-C-D06 (AB506319.1) / 91
16 / Akkermansia muciniphila ATCC BAA-835 strain (NR_074436.1) / 99
17 / Uncultured rumen bacterium 4C28d-12 (AB034126.1) / 92
18 / Uncultured rumen bacterium clone YRMCA8 (KC181125.1) / 91
19 / Uncultured bacterium clone R-C-D06 (AB506319.1) / 90
20 / Uncultured bacterium clone R-W-C05 (AB506388.1) / 92
21 / Uncultured Ruminococcaceae bacterium clone EMP_P11 (EU794122.1) / 96
22 / Uncultured rumen bacterium clone YRMCB89 (KC163086.1) / 97
23 / [Ruminococcus] gnavus canine oral taxon 145 clone PP024 (JN713312.1) / 94
24 / Uncultured bacterium clone SJTU_G_01_56 (EF405418.1) / 95
25 / Uncultured bacterium clone S68 (AB627696.1) / 91
26 / Uncultured bacterium clone C36 (AB627477.1) / 95
27 / Uncultured bacterium clone gir_aah94h02 (EU464144.1) / 97
28 / Uncultured bacterium gene clone A99 (AB494816.1) / 89
29 / Uncultured bacterium clone gir_aah94h02 (EU464144.1) / 96
30 / Akkermansia muciniphila ATCC BAA-835 strain (NR_074436.1) / 99
31 / Uncultured rumen bacterium clone U29-D04 (AB185775.1) / 90
32 / Uncultured bacterium clone ST-C-D07 (AB506529.1) / 87
33 / Uncultured bacterium clone p-3025-SwA5 (AF371528.1) / 99
34 / Uncultured bacterium clone FL_1aaa02a04 (EU775101.1) / 91
35 / Uncultured Erysipelotrichaceae bacterium clone EMP_E11 16S (EU794124.1) / 92
36 / Uncultured rumen bacterium clone YRMCA160 (KC163025.1) / 94
37 / Phascolarctos bacterium (JN713316.1) / 92
38 / Uncultured bacterium clone R-W-B10 (AB506382.1) / 91
39 / Uncultured bacterium clone J335 (GQ451315.1) / 96
40 / Akkermansia muciniphila ATCC BAA-835 (NR_074436.1) / 97
41 / Akkermansia muciniphila ATCC BAA-835 (NR_074436.1) / 97
42 / Uncultured rumen bacterium clone L7B_A04 (EU381588.1) / 93
43 / Uncultured bacterium clone C36 (AB627477.1) / 92
44 / Uncultured bacterium clone RL186_aan90b06 (DQ806301.1) / 94
45 / Uncultured organism clone ELU0090-T14-S-NI_000290 (HQ780832.1) / 88
46 / Uncultured Ruminococcaceae bacterium clone EMP_P11 (EU794122.1) / 92
47 / Uncultured bacterium clone T2WK15D76 (HQ716546.1) / 96
48 / Uncultured bacterium clone SJTU_G_01_56 16S (EF405418.1) / 94
49 / Uncultured bacterium clone G37 (FJ951883.1) / 97
50 / Uncultured bacterium clone Hda2-83 16S (JX095476.1) / 93
51 / Uncultured rumen bacterium clone YRMCA54 16S (KC162960.1) / 89
52 / Uncultured rumen bacterium clone U29-D09 (AB185779.1) / 88
53 / Uncultured bacterium clone 935 (AJ863522.1) / 91
54 / Uncultured bacterium clone R-9706 (FJ880656.1) / 96
55 / Uncultured bacterium clone S58 (AB627687.1) / 93
56 / Uncultured bacterium clone Thompsons83 (AY854361.1) / 91
57 / Uncultured bacterium clone J265 (GQ451273.1) / 93
58 / Uncultured bacterium clone SJTU_C_11_10 (EF404500.1) / 94
59 / Uncultured bacterium clone CNSL-03 (AB507592.1) / 93
60 / Uncultured bacterium clone B110 (HM124187.1) / 96
61 / Uncultured bacterium clone J39 (GQ451194.1) / 97
62 / Uncultured bacterium clone SJTU_G_01_56 (EF405418.1) / 96
63 / Uncultured bacterium clone p-1674-b3 (AF371779.1) / 94
64 / Uncultured bacterium clone B-56 (DQ115991.1) / 93
65 / Uncultured bacterium clone p-3025-SwA5 (AF371528.1) / 99
66 / Uncultured bacterium clone G-W-B07 (AB506217.1) / 92
67 / Uncultured bacterium clone AP10K.150 (AM275741.2) / 97
68 / Bacillus sp. M12K (AB735989.1) / 100
69 / Uncultured rumen bacterium clone U29-D09 (AB185779.1) / 89
70 / Uncultured bacterium clone J265 (GQ451273.1) / 97
71 / Uncultured bacterium clone J39 (GQ451194.1) / 97
Table SIV Results of BLAST search of the cultivable bacterial strains
1 / Bacillus atrophaeus 1942, complete genome (CP002207.1) / 99
2 / Bacillus megaterium strain PPB18 MB18 16S rRNA (HM771671.1) / 98
3 / Bacillus subtilis strain Bs18 16S ribosomal RNA (HQ267752.1) / 99
4 / Bacillus cereus strain L12 16S ribosomal RNA gene (HQ398861.1) / 99
5 / Arthrobacter globiformis strain CT402 16S rRNA (HQ455822.1) / 98
6 / Arthrobacter arilaitensis RE117 chromosome (HM191728.1) / 99
7 / Planococcus sp. enrichment culture clone (GU570653.1) / 99
Q-3 / Enterococcus gallinarum strain FMA288 16S rRNA (HQ721269.1) / 100
G-9 / Escherichia coli strain FUA1242 16S r RNA (HQ169124.1) / 100
Q-12 / Aerococcus sp. R-38529 partial 16S rRNA (FR691452.1) / 99
L-2 / Carnobacterium sp. aG53 gene for 16S rRNA (AB593334.1) / 100